Tissue Culture via Protocorm-like Bodies in an Orchids Hybrids <i>Paphiopedilum</i> SCBG Huihuang90
Beiyi Guo,
Hong Chen,
Yuying Yin,
Wei Wang,
Songjun Zeng
Affiliations
Beiyi Guo
Guangzhou Collaborative Innovation Center on Science-Tech of Ecology and Landscape, Guangzhou Landscape Plant Germplasm Resource Nursery, Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510540, China
Hong Chen
Guangzhou Collaborative Innovation Center on Science-Tech of Ecology and Landscape, Guangzhou Landscape Plant Germplasm Resource Nursery, Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510540, China
Yuying Yin
Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
Wei Wang
Guangzhou Collaborative Innovation Center on Science-Tech of Ecology and Landscape, Guangzhou Landscape Plant Germplasm Resource Nursery, Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510540, China
Songjun Zeng
Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
This study successfully established an efficient in vitro propagation system for Paphiopedilum SCBG Huihuang90 via protocorm-like body (PLB) formation from seed-derived calluses, PLB proliferation and differentiation, root induction and greenhouse acclimatization. Furthermore, 1/2 Murashige and Skoog (MS) + 0.025 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) was suitable for the proliferation of PLBs, and 1/2MS + 10% coconut water (CW, v/v) + 0.5 g/L activated carbon (AC) was suitable for PLB differentiation. PLBs at different developmental stages required different kinds of sugars. This study provided a reference for research on the propagation techniques of other Paphiopedilum.
