Efficiency of sandwich enzyme immunoassay for the diagnosis of larval echinococcus infection in cattle in the Republic of Azerbaijan

Abstract

The purpose of the research is to develop a sandwich enzyme-linked immunosorbent assay (ELISA) for laboratory diagnosis of echinococcosis in animals. Materials and methods. The diagnostic efficiency of the ELISA test system developed by us against cystic echinococcus infection in cattle was studied by comparing the obtained results with the data of a postmortem examination of animal carcasses. The sera from 80 animals delivered to the slaughterhouse from farms of different regions of the Republic of Azerbaijan were tested. Results and discussion. A positive ELISA test result was obtained in 42 animals, which was 52.5% of the total number of the animals studied. The postmortem diagnosis was confirmed in 32 animals, which was 40.0%. The comparison of the ELISA diagnostic efficiency with the postmortem examination data was 68.75%. Fasciola species were found in the bile ducts in 7 animals with a positive ELISA test response to echinococcosis and a negative diagnosis in the postmortem examination, and Cysticercus tenuicollis was found in one animal.

Keywords

• echinococcosis
• cattle
• liver
• lungs
• postmortem diagnostics
• sandwich elisa