Protocol for human placental mesenchymal stem cell therapy in a murine model of intra-abdominal infection of hypervirulent Klebsiella

Li-Tzu Wang; Sheng-Kang Chiu; Wei Lee; L. Kristopher Siu; Ko-Jiunn Liu; Men-Luh Yen; B. Linju Yen

STAR Protocols (Mar 2021)

Protocol for human placental mesenchymal stem cell therapy in a murine model of intra-abdominal infection of hypervirulent Klebsiella

Li-Tzu Wang,
Sheng-Kang Chiu,
Wei Lee,
L. Kristopher Siu,
Ko-Jiunn Liu,
Men-Luh Yen,
B. Linju Yen

Affiliations

Li-Tzu Wang: Department of Obstetrics & Gynecology, National Taiwan University (NTU) Hospital & College of Medicine, NTU, Taipei 100, Taiwan
Sheng-Kang Chiu: Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan
Wei Lee: Regenerative Medicine Research Group, Institute of Cellular & System Medicine, National Health Research Institutes (NHRI), Zhunan 350, Taiwan
L. Kristopher Siu: Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan; National Institute of Infectious Diseases & Vaccinology, NHRI, Zhunan 350, Taiwan; Graduate Institute of Basic Medical Science, China Medical University, Taichung 404, Taiwan
Ko-Jiunn Liu: National Institute of Cancer Research, NHRI, Zhunan 350, Taiwan; Institute of Clinical Pharmacy and Pharmaceutical Sciences, National Cheng Kung University, Tainan 701, Taiwan; School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei 110, Taiwan
Men-Luh Yen: Department of Obstetrics & Gynecology, National Taiwan University (NTU) Hospital & College of Medicine, NTU, Taipei 100, Taiwan; Corresponding author
B. Linju Yen: Regenerative Medicine Research Group, Institute of Cellular & System Medicine, National Health Research Institutes (NHRI), Zhunan 350, Taiwan; Department of Obstetrics & Gynecology, Cathay General Hospital Shiji, New Taipei City 221, Taiwan; Corresponding author

Journal volume & issue: Vol. 2, no. 1
p. 100337

Abstract

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Summary: Hypervirulent Klebsiella pneumoniae (hvKP) strains cause extra-pulmonary infections such as intra-abdominal infection (IAI) even in healthy individuals due to its resistance to polymorphonuclear neutrophil (PMN) killing and a high incidence of multidrug resistance. To assess whether human placental mesenchymal stem cell (PMSC) therapy can be an effective treatment option, we established a murine model of hvKP-IAI to evaluate immune cell modulation and bacterial clearance for this highly lethal infection. This protocol can rapidly assess potential therapies for severe bacterial IAIs.For complete details on the use and execution of this protocol, please refer to Wang et al. (2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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