Dynamics of cell proportions in Axolotl’s brain during development and regeneration

Abstract

Exploring Axolotl’s regenerative capacities, encompassing the remarkable regeneration of telencephalon, has been pivotal in acquiring foundational insights into biomedicine and neuro-regeneration. Our study aimed to explore gene expression variability in the axolotl telencephalon during development and regeneration. We generated signature matrices for 12 individual gene expression datasets through CIBERSORTx, combining them with raw bulk RNA sequencing data from axolotl brain samples. Our analysis revealed dynamic changes in cell type proportions, including an increased representation of median spinal neurons (MSNs) during regeneration compared to developmental and control stages. While this finding does not establish a direct functional role for MSNs in brain regeneration, it highlights a notable pattern that warrants further experimental validation. Future studies are needed to explore the potential interactions of MSNs with ependymal glial cells (EGCs) and neuroblasts, as well as their contributions to the regenerative process in amphibians.

Keywords

• Axolotl
• Telencephalon
• Regeneration
• Development
• Single-cell
• CIBERSORTx