Frontiers in Microbiology (Jun 2021)
Porcine RIG-I and MDA5 Signaling CARD Domains Exert Similar Antiviral Function Against Different Viruses
- Shuangjie Li,
- Shuangjie Li,
- Shuangjie Li,
- Shuangjie Li,
- Qi Shao,
- Qi Shao,
- Qi Shao,
- Qi Shao,
- Yuanyuan Zhu,
- Yuanyuan Zhu,
- Yuanyuan Zhu,
- Yuanyuan Zhu,
- Xingyu Ji,
- Xingyu Ji,
- Xingyu Ji,
- Xingyu Ji,
- Jia Luo,
- Jia Luo,
- Jia Luo,
- Jia Luo,
- Yulin Xu,
- Yulin Xu,
- Yulin Xu,
- Yulin Xu,
- Xueliang Liu,
- Xueliang Liu,
- Xueliang Liu,
- Xueliang Liu,
- Wanglong Zheng,
- Wanglong Zheng,
- Wanglong Zheng,
- Wanglong Zheng,
- Nanhua Chen,
- Nanhua Chen,
- Nanhua Chen,
- Nanhua Chen,
- François Meurens,
- François Meurens,
- Jianzhong Zhu,
- Jianzhong Zhu,
- Jianzhong Zhu,
- Jianzhong Zhu
Affiliations
- Shuangjie Li
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Shuangjie Li
- College of Veterinary Medicine, Yangzhou University, Yangzhou, China
- Shuangjie Li
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Shuangjie Li
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Qi Shao
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Qi Shao
- College of Veterinary Medicine, Yangzhou University, Yangzhou, China
- Qi Shao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Qi Shao
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Yuanyuan Zhu
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Yuanyuan Zhu
- College of Veterinary Medicine, Yangzhou University, Yangzhou, China
- Yuanyuan Zhu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Yuanyuan Zhu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Xingyu Ji
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Xingyu Ji
- College of Veterinary Medicine, Yangzhou University, Yangzhou, China
- Xingyu Ji
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Xingyu Ji
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Jia Luo
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Jia Luo
- College of Veterinary Medicine, Yangzhou University, Yangzhou, China
- Jia Luo
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Jia Luo
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Yulin Xu
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Yulin Xu
- College of Veterinary Medicine, Yangzhou University, Yangzhou, China
- Yulin Xu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Yulin Xu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Xueliang Liu
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Xueliang Liu
- College of Veterinary Medicine, Yangzhou University, Yangzhou, China
- Xueliang Liu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Xueliang Liu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Wanglong Zheng
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Wanglong Zheng
- College of Veterinary Medicine, Yangzhou University, Yangzhou, China
- Wanglong Zheng
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Wanglong Zheng
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Nanhua Chen
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Nanhua Chen
- College of Veterinary Medicine, Yangzhou University, Yangzhou, China
- Nanhua Chen
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Nanhua Chen
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- François Meurens
- BIOEPAR, INRAE, Oniris, Nantes, France
- François Meurens
- Department of Veterinary Microbiology and Immunology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada
- Jianzhong Zhu
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Jianzhong Zhu
- College of Veterinary Medicine, Yangzhou University, Yangzhou, China
- Jianzhong Zhu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Jianzhong Zhu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- DOI
- https://doi.org/10.3389/fmicb.2021.677634
- Journal volume & issue
-
Vol. 12
Abstract
The RIG-I-like receptors (RLRs) RIG-I and MDA5 play critical roles in sensing and fighting viral infections. Although RIG-I and MDA5 have similar molecular structures, these two receptors have distinct features during activation. Further, the signaling domains of the N terminal CARD domains (CARDs) in RIG-I and MDA5 share poor similarity. Therefore, we wonder whether the CARDs of RIG-I and MDA5 play similar roles in signaling and antiviral function. Here we expressed porcine RIG-I and MDA5 CARDs in 293T cells and porcine alveolar macrophages and found that MDA5 CARDs exhibit higher expression and stronger signaling activity than RIG-I CARDs. Nevertheless, both RIG-I and MDA5 CARDs exert comparable antiviral function against several viruses. Transcriptome analysis showed that MDA5 CARDs are more effective in regulating downstream genes. However, in the presence of virus, both RIG-I and MDA5 CARDs exhibit similar effects on downstream gene transcriptions, reflecting their antiviral function.
Keywords