Lipidomic Adaptations in White and Brown Adipose Tissue in Response to Exercise Demonstrate Molecular Species-Specific Remodeling
Francis J. May,
Lisa A. Baer,
Adam C. Lehnig,
Kawai So,
Emily Y. Chen,
Fei Gao,
Niven R. Narain,
Liubov Gushchina,
Aubrey Rose,
Andrea I. Doseff,
Michael A. Kiebish,
Laurie J. Goodyear,
Kristin I. Stanford
Affiliations
Francis J. May
Department of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA
Lisa A. Baer
Department of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA
Adam C. Lehnig
Department of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA
Kawai So
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Boston, MA 02215, USA
Emily Y. Chen
BERG Health, Framingham, MA 01701, USA
Fei Gao
BERG Health, Framingham, MA 01701, USA
Niven R. Narain
BERG Health, Framingham, MA 01701, USA
Liubov Gushchina
Department of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA
Aubrey Rose
Department of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA
Andrea I. Doseff
Department of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA
Michael A. Kiebish
BERG Health, Framingham, MA 01701, USA
Laurie J. Goodyear
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Boston, MA 02215, USA
Kristin I. Stanford
Department of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA
Exercise improves whole-body metabolic health through adaptations to various tissues, including adipose tissue, but the effects of exercise training on the lipidome of white adipose tissue (WAT) and brown adipose tissue (BAT) are unknown. Here, we utilize MS/MSALL shotgun lipidomics to determine the molecular signatures of exercise-induced adaptations to subcutaneous WAT (scWAT) and BAT. Three weeks of exercise training decrease specific molecular species of phosphatidic acid (PA), phosphatidylcholines (PC), phosphatidylethanolamines (PE), and phosphatidylserines (PS) in scWAT and increase specific molecular species of PC and PE in BAT. Exercise also decreases most triacylglycerols (TAGs) in scWAT and BAT. In summary, exercise-induced changes to the scWAT and BAT lipidome are highly specific to certain molecular lipid species, indicating that changes in tissue lipid content reflect selective remodeling in scWAT and BAT of both phospholipids and glycerol lipids in response to exercise training, thus providing a comprehensive resource for future studies of lipid metabolism pathways.
