Frontiers in Veterinary Science (Mar 2024)
Association between polymorphisms in NOBOX and litter size traits in Xiangsu pigs
- Jiajin Huang,
- Jiajin Huang,
- Jiajin Huang,
- Yong Ruan,
- Yong Ruan,
- Yong Ruan,
- Meimei Xiao,
- Meimei Xiao,
- Meimei Xiao,
- Lingang Dai,
- Lingang Dai,
- Lingang Dai,
- Chuanmei Jiang,
- Chuanmei Jiang,
- Chuanmei Jiang,
- Jifeng Li,
- Jifeng Li,
- Jifeng Li,
- Jiali Xu,
- Jiali Xu,
- Jiali Xu,
- Xiang Chen,
- Xiang Chen,
- Xiang Chen,
- Houqiang Xu,
- Houqiang Xu,
- Houqiang Xu
Affiliations
- Jiajin Huang
- Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China
- Jiajin Huang
- Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang, China
- Jiajin Huang
- College of Animal Science, Guizhou University, Guiyang, China
- Yong Ruan
- Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China
- Yong Ruan
- Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang, China
- Yong Ruan
- College of Animal Science, Guizhou University, Guiyang, China
- Meimei Xiao
- Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China
- Meimei Xiao
- Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang, China
- Meimei Xiao
- College of Animal Science, Guizhou University, Guiyang, China
- Lingang Dai
- Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China
- Lingang Dai
- Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang, China
- Lingang Dai
- College of Animal Science, Guizhou University, Guiyang, China
- Chuanmei Jiang
- Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China
- Chuanmei Jiang
- Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang, China
- Chuanmei Jiang
- College of Animal Science, Guizhou University, Guiyang, China
- Jifeng Li
- Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China
- Jifeng Li
- Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang, China
- Jifeng Li
- College of Animal Science, Guizhou University, Guiyang, China
- Jiali Xu
- Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China
- Jiali Xu
- Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang, China
- Jiali Xu
- College of Animal Science, Guizhou University, Guiyang, China
- Xiang Chen
- Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China
- Xiang Chen
- Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang, China
- Xiang Chen
- College of Animal Science, Guizhou University, Guiyang, China
- Houqiang Xu
- Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China
- Houqiang Xu
- Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang, China
- Houqiang Xu
- College of Animal Science, Guizhou University, Guiyang, China
- DOI
- https://doi.org/10.3389/fvets.2024.1359312
- Journal volume & issue
-
Vol. 11
Abstract
The newborn ovary homeobox gene (NOBOX) regulates ovarian and early oocyte development, and thus plays an essential role in reproduction. In this study, the mRNA expression level and single nucleotide polymorphism (SNP) of NOBOX in various tissues of Xiangsu pigs were studied to explore the relationship between its polymorphism and litter size traits. Also, bioinformatics was used to evaluate the effects of missense substitutions on protein structure and function. The results revealed that NOBOX is preferentially expressed in the ovary. Six mutations were detected in the NOBOX sequence, including g.1624 T>C, g.1858 G>A, g.2770 G>A, g.2821 A>G, g.5659 A>G, and g.6025 T>A, of which g.1858 G>A was a missense mutation. However, only g.1858 G>A, g.5659 A>G, and g.6025 T>A were significantly associated with litter size traits (p < 0.05). Further prediction of the effect of the missense mutation g.1858 G>A on protein function revealed that p.V82M is a non-conservative mutation that significantly reduces protein stability and thus alters protein function. Overall, these findings suggest that NOBOX polymorphism is closely related to the litter size of Xiangsu pigs, which may provide new insights into pig breeding.
Keywords
