Abscisic acid metabolism pathways differ between grapevine species, leaves, and roots during water deficit

Abstract

Abscisic acid (ABA) metabolism is complex involving biosynthesis, conjugation, and catabolism. Differences in ABA metabolism (ABA, ABA-related metabolites, and transcripts) in response to water deficit (WD) were detected in leaves and roots of four Vitis species differing in drought tolerance: Vitis vinifera (Cabernet Sauvignon), Vitis champinii (Ramsey), Vitis riparia (Riparia Gloire), and Vitis vinifera x girdiana (SC2). Concentrations of ABA and ABA-related metabolites increased after moderate or severe WD depending on species, organ, and time. Differences in ABA, glycosylated-ABA, and ABA catabolite concentrations, as well as previously investigated related transcript abundances, revealed differences in ABA metabolism pathways among the species and organs. NCED3 was a key gene in the WD response of leaves and roots of all species. NCED3 transcript abundance and ABA concentration in drought-tolerant Ramsey increased earlier and to a greater extent than other species. These species provide informative genetic resources to study ABA metabolism and drought tolerance further.

Keywords

• Abscisic acid
• water deficit
• nine-cis-epoxycarotenoid dioxygenase
• grapevine