Immunological Medicine (Apr 2024)

Possible correlation between serum interleukin-8 levels and the activity of myositis in anti-NXP2 antibody-positive dermatomyositis

  • Risa Konishi,
  • Yuki Ichimura,
  • Ryota Tanaka,
  • Hanako Miyahara,
  • Mari Okune,
  • Masahide Miyamoto,
  • Monami Hara,
  • Atsushi Iwabuchi,
  • Hidetoshi Takada,
  • Yasuo Nakagishi,
  • Mao Mizuta,
  • Shuya Kaneko,
  • Masaki Shimizu,
  • Tomohiro Morio,
  • Ichizo Nishino,
  • Toshifumi Nomura,
  • Naoko Okiyama

DOI
https://doi.org/10.1080/25785826.2023.2300553
Journal volume & issue
Vol. 47, no. 2
pp. 100 – 105

Abstract

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AbstractAnti-nuclear matrix protein 2 (NXP2) antibody-positive dermatomyositis (DM) is characterized by extensive and severe myositis. In this study, we evaluated which cytokines/chemokines involved with the activity of the myositis. We performed quantitative immunoassays using the MILLIPLEX® Multiplex Assays Using Luminex to evaluate serum levels of interferon-γ, interleukin (IL)-1β, IL-6, IL-8, IL-12p40, and tumor necrosis factor-α in samples collected over time from a 9-year-old female with anti-NXP2 antibody-positive DM. In our case, the serum level of IL-8 was elevated when the myositis worsened, and decreased in accordance with the improvement of myositis, suggesting that the serum IL-8 levels were correlated with the myositis activity. Serum levels of IL-8 in samples from five patients with anti-NXP2 antibody-positive DM and five patients with anti-transcriptional intermediary factor 1γ (TIF1γ) antibody-positive DM without both interstitial lung disease (ILD) and malignancy before starting treatments, along with five healthy controls, were also evaluate by an enzyme-linked immunosorbent assay. Serum IL-8 levels were significantly elevated in anti-NXP2 or anti-TIF1γ antibody-positive DM patients with myositis but not ILD, than healthy controls. It was suggested that serum levels of IL-8 correlate with the activity of myositis in DM including anti-NXP2 antibody-positive DM.

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