Effect of long non-coding RNA DUXAP8 on proliferation, cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p

Abstract

Objective To investigate the effects of long non-coding RNA DUXAP8 on the proliferation, cell cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p. Methods The expression of DUXAP8 and miR-4677-3p in ovarian cancer tissues and cell lines (SKOV3, OV90 and CaOV3) was detected by RT-qPCR. Then SKOV3 cells were divided into 6 groups, namely, si-NC group, si-DUXAP8 group, miR-NC group, miR-4677-3p group, si-DUXAP8+anti-miR-NC group and si-DUXAP8+anti-miR-4677-3p group. CCK-8 assay and clone formation assay were adopted to detect cell viability, flow cytometry to observe cell apoptosis and cycle, Western blotting to measure the expression levels of Cyclin D1, Bcl-2 and Bax, and dual luciferase reporter assay system to analyze the relationship of DUXAP8 and miR-4677-3p. Results When compared with adjacent tissues and normal ovarian epithelial cells, the expression level of DUXAP8 was significantly lower in ovarian cancer tissues (0.98±0.25 vs 4.14±0.72) and that of SKOV3, OV90, CaOV3 was obviously higher (1.03±0.08 vs 3.26±0.33、2.75±0.29、2.54±0.26, all P < 0.05). Interference with DUXAP8 or overexpression of miR-4677-3p inhibited the proliferation, reduced the rate of apoptosis, and arrested the cell cycle of ovarian cancer cells (P < 0.05). DUXAP8 regulated the expression of miR-4677-3p, and inhibiting miR-4677-3p partially restore and interfere with the effects of DUXAP8 on the proliferation, cycle and apoptosis of ovarian cancer cells (P < 0.05). Conclusion Interference with DUXAP8 can inhibit the proliferation of ovarian cancer cells, induce cell apoptosis, and block cell cycle, the mechanism may be related to the up-regulation of miR-4677-3p.

Keywords

• duxap8
• mir-4677-3p
• ovarian cancer cells
• proliferation
• cycle
• apoptosis