Bio-Protocol (Jul 2018)

Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL)

  • Anna Worthmann,
  • Clara John,
  • Joerg Heeren

DOI
https://doi.org/10.21769/bioprotoc.2916
Journal volume & issue
Vol. 8, no. 13

Abstract

Read online

The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice housed at different ambient temperatures