Frontiers in Cardiovascular Medicine (May 2023)

A plasma proteomic approach in patients with heart failure after acute myocardial infarction: insights into the pathogenesis and progression of the disease

  • Yan Liu,
  • Da Huang,
  • Zhile Li,
  • LiuFang Zhou,
  • Tuan Cen,
  • Baomin Wei,
  • Liuqing Wei,
  • Hongying Wu,
  • Liye Su,
  • Liye Su,
  • Suren R. Sooranna,
  • Suren R. Sooranna,
  • Xinshou Pan,
  • ZhaoHe Huang,
  • ZhaoHe Huang

DOI
https://doi.org/10.3389/fcvm.2023.1153625
Journal volume & issue
Vol. 10

Abstract

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AimsThe pathogenesis of disease progression targets for patients with heart failure after acute myocardial infarction was investigated by using plasma proteomics.MethodsThe plasma proteomes of acute myocardial infarction patients with (MI-HF) and without (MI-WHF) heart failure were compared. Each group consisted of 10 patients who were matched for age and sex. The peptides were analyzed by 2-dimensional liquid chromatography coupled to tandem mass spectrometry in a high definition mode. Parallel reaction monitoring (PRM) verified the selected target proteins.ResultsWe identified and quantified 2,589 and 2,222 proteins, respectively, and found 117 differentially expressed proteins (DEPs) (≥1.5-fold), when the MI-HF and MI-WHF groups were compared. Of these 51 and 66 were significantly up-regulated and down-regulated, respectively. The significant DEPs was subjected to protein–protein interaction network analysis which revealed a central role of the NF-κB signaling pathway in the MI-HF patients. PRM verified that MB, DIAPH1, VNN1, GOT2, SLC4A1, CRP, CKM, SOD3, F7, DLD, PGAM2, GOT1, UBA7 and HYOU1 were 14 proteins which were highly expressed in MI-HF patients.ConclusionsThese findings showed a group of proteins related to the NF-κB signaling pathway in the pathogenesis of patients with poor outcomes after experiencing MI-HF. These proteins may be useful candidate markers for the diagnosis of MI-HF as well as help to elucidate the pathophysiology of this major cause of mortality in older patients.

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