The Regulatory Role of <i>Myomaker</i> in the Muscle Growth of the Chinese Perch (<i>Siniperca chuatsi</i>)
Wei Zeng,
Yangyang Meng,
Lingtao Nie,
Congyi Cheng,
Zexia Gao,
Lusha Liu,
Xin Zhu,
Wuying Chu
Affiliations
Wei Zeng
Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Hunan Engineering Technology Research Center for Amphibian and Reptile Resource Protection and Product Processing, College of Biological and Chemical Engineering, Changsha University, Changsha 410022, China
Yangyang Meng
Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Hunan Engineering Technology Research Center for Amphibian and Reptile Resource Protection and Product Processing, College of Biological and Chemical Engineering, Changsha University, Changsha 410022, China
Lingtao Nie
Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Hunan Engineering Technology Research Center for Amphibian and Reptile Resource Protection and Product Processing, College of Biological and Chemical Engineering, Changsha University, Changsha 410022, China
Congyi Cheng
Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Hunan Engineering Technology Research Center for Amphibian and Reptile Resource Protection and Product Processing, College of Biological and Chemical Engineering, Changsha University, Changsha 410022, China
Zexia Gao
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Wuhan 430070, China
Lusha Liu
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Wuhan 430070, China
Xin Zhu
Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Hunan Engineering Technology Research Center for Amphibian and Reptile Resource Protection and Product Processing, College of Biological and Chemical Engineering, Changsha University, Changsha 410022, China
Wuying Chu
Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Hunan Engineering Technology Research Center for Amphibian and Reptile Resource Protection and Product Processing, College of Biological and Chemical Engineering, Changsha University, Changsha 410022, China
The fusion of myoblasts is a crucial stage in the growth and development of skeletal muscle. Myomaker is an important myoblast fusion factor that plays a crucial role in regulating myoblast fusion. However, the function of Myomaker in economic fish during posthatching has been poorly studied. In this study, we found that the expression of Myomaker in the fast muscle of Chinese perch (Siniperca chuatsi) was higher than that in other tissues. To determine the function of Myomaker in fast muscle, Myomaker-siRNA was used to knockdown Myomaker in Chinese perch and the effect on muscle growth was determined. The results showed that the growth of Chinese perch was significantly decreased in the Myomaker-siRNA group. Furthermore, both the diameter of muscle fibers and the number of nuclei in single muscle fibers were significantly reduced in the Myomaker-siRNA group, whereas there was no significant difference in the number of BrdU-positive cells (proliferating cells) between the control and the Myomaker-siRNA groups. Together, these findings indicate that Myomaker may regulate growth of fast muscle in Chinese perch juveniles by promoting myoblast fusion rather than proliferation.
