Generation and characterization of a human induced pluripotent stem cell line (XWHNi001-A) derived from an Alzheimer's disease patient with mutation in the APP gene

Cuibai Wei; Xinliang Lu; Qi Zou; Shuting Gong; Yufei Chen; Jihui Lyu; Jianping Jia

Stem Cell Research (Apr 2022)

Generation and characterization of a human induced pluripotent stem cell line (XWHNi001-A) derived from an Alzheimer's disease patient with mutation in the APP gene

Cuibai Wei,
Xinliang Lu,
Qi Zou,
Shuting Gong,
Yufei Chen,
Jihui Lyu,
Jianping Jia

Affiliations

Cuibai Wei: Innovation Center for Neurological Disorders, Xuan Wu Hospital, Capital Medical University, China; Department of Neurology, Xuan Wu Hospital, Capital Medical University, Beijing, China; Corresponding author at: Innovation center for neurological disorders & Department of Neurology, Xuanwu Hospital, Capital Medical University, 45 Changchun Street, Beijing 100053, China. Tel.: +86 10 83199456; fax: +86 10 83171070.
Xinliang Lu: Innovation Center for Neurological Disorders, Xuan Wu Hospital, Capital Medical University, China; School of Biological Science and Medical Engineering, Beihang University, Beijing, China
Qi Zou: Innovation Center for Neurological Disorders, Xuan Wu Hospital, Capital Medical University, China; College of Integrated Traditional Chinese and Western Medicine, Changchun University of Chinese Medicine, Changchun, China
Shuting Gong: Innovation Center for Neurological Disorders, Xuan Wu Hospital, Capital Medical University, China; School of Biological Science and Medical Engineering, Beihang University, Beijing, China
Yufei Chen: Innovation Center for Neurological Disorders, Xuan Wu Hospital, Capital Medical University, China; Department of Neurology, Xuan Wu Hospital, Capital Medical University, Beijing, China
Jihui Lyu: Beijing Geriatric Hospital, Beijing, China
Jianping Jia: Innovation Center for Neurological Disorders, Xuan Wu Hospital, Capital Medical University, China; Department of Neurology, Xuan Wu Hospital, Capital Medical University, Beijing, China

Journal volume & issue: Vol. 60
p. 102690

Abstract

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Mutations in the APP gene are popularly known as the second cause trigger the familial Alzheimer's disease (AD). We generated a human induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells isolated from an AD patient using non-integrative Sendai virus. The iPSC line highly expresses pluripotency markers, has the capacity to differentiate into the normal teratoma tissue, retains the APP mutation, and displays the normal karyotype. The iPSC line will provide a useful resource to study the pathogenesis and drugs screening of AD.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal