Quantitative live cell imaging reveals influenza virus manipulation of Rab11A transport through reduced dynein association

Amar R. Bhagwat; Valerie Le Sage; Eric Nturibi; Katarzyna Kulej; Jennifer Jones; Min Guo; Eui Tae Kim; Benjamin A. Garcia; Matthew D. Weitzman; Hari Shroff; Seema S. Lakdawala

doi:10.1038/s41467-019-13838-3

Nature Communications (Jan 2020)

Quantitative live cell imaging reveals influenza virus manipulation of Rab11A transport through reduced dynein association

Amar R. Bhagwat,
Valerie Le Sage,
Eric Nturibi,
Katarzyna Kulej,
Jennifer Jones,
Min Guo,
Eui Tae Kim,
Benjamin A. Garcia,
Matthew D. Weitzman,
Hari Shroff,
Seema S. Lakdawala

Affiliations

Amar R. Bhagwat: Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine
Valerie Le Sage: Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine
Eric Nturibi: Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine
Katarzyna Kulej: The Children’s Hospital of Philadelphia Research Institute
Jennifer Jones: Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine
Min Guo: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
Eui Tae Kim: The Children’s Hospital of Philadelphia Research Institute
Benjamin A. Garcia: Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine
Matthew D. Weitzman: The Children’s Hospital of Philadelphia Research Institute
Hari Shroff: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
Seema S. Lakdawala: Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine

DOI: https://doi.org/10.1038/s41467-019-13838-3
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 14

Abstract

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Here, using high spatiotemporal resolution light-sheet and fluorescence microscopy, the authors investigate the role of cytoskeletal components on the intracellular transport of Rab11A and influenza virus (IAV) vRNP), and show a preference for Rab11A movement along microtubules that is not essential for IAV vRNP transport.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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