Innovative Biosystems and Bioengineering (Apr 2019)
Experimental and Theoretical Substantiation of the Express Method Development for Detection of Enteroviruses in Water by Surface Plasmon Resonance Method
Abstract
Background. As a result of anthropogenic pollution and number of other factors, in drinking water can be present some viruses such as enterovirus and other pathogens that can be transmitted by water. Enteroviruses are capable of causing lesions of organs and systems of varying severity in humans, manifested by a number of clinical symptoms. For the detection of viruses appropriate research methods were developed, but they take a lot of time and require special reagents and equipment, that is significant drawback of sanitary-virological studies of drinking water quality. Objective. The aim of the paper is to study the general possibility of using a surface plasmon resonance (SPR) phenomenon for detection of enteroviruses in water samples (swine Teschovirus A as an example) and to create on its basis a new express diagnostic method. Methods. For implementation of a method for rapid detection of viral diseases pathogens in water by using SPR, the Plasmon-6 device was developed at the Institute of V.E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine. The research on the interaction of antigen and specific serum in various buffers was held at the State Scientific Control Institute of Biotechnology and Strains of Microorganisms. Results. A general possibility of using SPR to detect enteroviruses in distilled and drinking water (swine Teschovirus A as an example) was theoretically substantiated and experimentally proved, and the express method for their detection was developed. It is shown that upon detection of pathogen in distilled water, the response of the SPR sensor is 1.57 times higher than in drinking water, which may be due to the influence of ionic composition in water on the interaction of the antigen with the antibody. The developed method allows real-time monitoring of changes in the test environment, significantly reduces the duration of enteroviruses detection and does not require the use of expensive nutrient media and reagents. Сonclusions. The possibility of using the SPR method and the "Plasmon-6" device implementing this method for express detection of enteroviruses in distilled and drinking water was theoretically substantiated and experimentally proved.
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