Effects of Advanced Age, Pituitary Pars Intermedia Dysfunction and Insulin Dysregulation on Serum Antioxidant Markers in Horses
Agnieszka Żak,
Natalia Siwińska,
Elżbieta Chełmecka,
Barbara Bażanów,
Ewa Romuk,
Amanda Adams,
Artur Niedźwiedź,
Dominika Stygar
Affiliations
Agnieszka Żak
Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, University of Environmental and Life Sciences, 50-375 Wroclaw, Poland
Natalia Siwińska
Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, 50-366 Wroclaw, Poland
Elżbieta Chełmecka
Department of Statistics, Department of Instrumental Analysis, Faculty of Pharmaceutical Sciences in Sosnowiec Medical University of Silesia, 40-055 Katowice, Poland
Barbara Bażanów
Department of Pathology, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland
Ewa Romuk
Department of Biochemistry, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 41-808 Katowice, Poland
Amanda Adams
Department of Veterinary Science, MH Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA
Artur Niedźwiedź
Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, 50-366 Wroclaw, Poland
Dominika Stygar
Department of Physiology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia, 41-808 Katowice, Poland
The study aims to assess the impact of age, pituitary pars intermedia dysfunction (PPID) and insulin dysregulation (ID) in horses on selected oxidative stress markers. The study includes 32 horses, divided into three groups: “young” adult group (aged 8–16 years old) “geriatric” group (aged 18–24 years old) and the “PPID” group (aged 15–31 years old). The PPID group was further divided into two subgroups: PPID ID+ and PPID ID− based on presence or absence of ID. We measured serum antioxidant stress markers in all horses: total oxidant status (TOS), total antioxidant capacity (TAC), ceruloplasmin (CER), lipofuscin (LPS), malondialdehyde (MDA) and thiols concentrations (containing sulfhydryl group -SH) as well as enzymatic systems: total superoxide dismutase (SOD), cytoplasmic SOD (CuZnSOD), mitochondrial SOD activity (MnSOD). Total serum thiols were significantly lower in the geriatric group and in the PPID group compared to the young group. The MnSOD concentration was higher in the PPID ID+ group compared to the PPID ID−. LPS and MDA concentrations were lower in the PPID ID+ group compared to the PPID ID− group. In the selected study groups of horses, older age, the presence of PPID and ID in the case of PPID had no effect on the studied oxidative stress markers.
