Occurrence, Distribution and Biological variability of Zucchini Yellow Mosaic Virus in cucurbits of Khuzestan province, South west of Iran

Abstract

ZYMV is one of the most important plant viruses that cause economical damage in cucurbits. The symptoms of ZYMV in different cucurbits include stunting, yellowing, mottling, severe mosaic, leaf and fruit deformation, blistering and shoe string. Investigation on occurrence of this virus, in Khuzestan province was carried out in November 2009, April and May 2010 by collecting cucurbits samples from different cucurbits fields. After DAS-ELISA test, ZYMV was maintained in squash. Then total RNA were extracted and were tested by RT-PCR. Using RT-PCR, fragments belonging to N-terminal of coat protein and C-terminal of nuclear inclusion bodies were replicated. PCR product for investigation of replication was loaded in 1% agarose gel. From seven regions in Khuzestan, 175 leaf samples showing different symptoms (yellowing, mosaic, deformation and blistering) were collected. Seventy one samples out of total samples (175 samples) showed ZYMV infection. Occurrence of Zucchini Yellow Mosaic Virus in Khuzestan province was confirmed, using serological and RT-PCR tests. Infection of ZYMV in Khuzestan province (40.5%) is higher than the average of Iran’s infection (38%). This article is first report of occurrence ZYMV in different regions of Khuzestan province except Dezful.

Keywords

• ZYMV, DAS-ELISA, Distribution, Khuzestan province, RT-PCR