Evaluation of the cardioprotective activity of summer savory (Satureja hortensis L.) extract in experimental rats with Isoproterenol-induced myocardial infarction

Abstract

Background: Myocardial infarction (MI) is a serious heart ailment that requires cutting-edge remedies for effective treatment. This study examines the possible cardioprotective effects of a hydroalcoholic extract from Satureja hortensis L. (HASH) in rats with isoproterenol (ISO) induced myocardial infarction. Methods: The rats were pretreated orally with hydroalcoholic extract of Satureja hortensis low (200 mg/kg), high dose (400 mg/kg), and metoprolol (10 mg/kg), in their respective groups for 30 days, followed by two subcutaneous doses of isoproterenol (ISO). Blood was obtained to measure biochemicals such as creatine kinase-MB (CK-MB), Troponin I, Alanine transaminase (ALT), Aspartate transaminase (AST), and lactate dehydrogenase (LDH), and an electrocardiogram (ECG) of a rat was recorded after the experiment. Myocardial integrity was examined histopathologically, and malondialdehyde (MDA) levels and antioxidant enzyme concentrations were assessed using heart tissue homogenate. Results: Rats with myocardial infarction caused by ISO displayed significantly (P < 0.001) augmented CK-MB, Troponin I, LDH, AST, and ALT levels with aberrant ECG patterns, and alteration in cardiac mass as well as enhaced oxidative stress marker levels. Besides, biochemical results were validated by myocardium histology. Pre-treatment of animals with a high dose (400 mg/kg) of HASH or standard cardioprotective agent (metoprolol) prevented the ISO-induced alterations in the above parameters to a significant (P < 0.01) level as well as metabolic derangements and functional alterations. Conclusions: Our research suggests that HASH pre-treatment has cardioprotective action and can prevent myocardial toxicity caused by ISO. Therefore, Satureja hortensis L. extract might be a promising therapeutic plant that can be further investigated for potential cardioprotective properties.

Keywords

• Drug screening
• Satureja hortensis L
• Isoproterenol
• Metoprolol
• Cardiac markers enzymes
• Antioxidants