Viability modulation of cells immobilized in porous TiNi-based alloy scaffold under infrared and ultraviolet radiation

Abstract

Cellular response to electromagnetic radiation depends on many factors including the microenvironment of cell. Different cells (spleen, bone marrow, and Ehrlich's adenocarcinoma), respond to the studied types of low-intensity radiation. Exposure to infrared (IR) and ultraviolet (UV) radiation of low intensity leads to significant change in the number of viable cells. Effects of low-intensity infrared (IR) and ultraviolet (UV) radiation on the number of viable cells were evaluated against the control group in which cells were exposed to natural daylight. The results showed that IR irradiation led to a 4.6-, 2.5-, and 1.3-fold increase in viable Ehrlich tumor, bone marrow, and spleen cells, respectively, while UV exposure led to a 3.9-, 1.5-, and 1.2-fold increase, respectively. The data show that the extracellular environment of bone marrow, tumor and spleen cell populations affects their viability and proliferative potency in porous TiNi-based scaffolds. IR- and UV irradiation of cell cultures immobilized in the scaffold affects the cell viability in populations of bone marrow, tumor, and spleen cells. In case of IR irradiation, cell viability was significantly improved, at the same time UV irradiation suppressed cell proliferation activity. The effect of IR irradiation can be used to resuscitate the cell area. The effect of UV irradiation can be used to destroy residual tumor lesions or other pathological cell populations.

Keywords

• Скаффолд
• Экстрацеллюлярный матрикс
• Инкубатор-носитель клеток
• Пористый никелид титана
• Инфракрасное и ультрафиолетовое излучения