Genome-wide maps of nucleolus interactions reveal distinct layers of repressive chromatin domains

Cristiana Bersaglieri; Jelena Kresoja-Rakic; Shivani Gupta; Dominik Bär; Rostyslav Kuzyakiv; Martina Panatta; Raffaella Santoro

doi:10.1038/s41467-022-29146-2

Nature Communications (Mar 2022)

Genome-wide maps of nucleolus interactions reveal distinct layers of repressive chromatin domains

Cristiana Bersaglieri,
Jelena Kresoja-Rakic,
Shivani Gupta,
Dominik Bär,
Rostyslav Kuzyakiv,
Martina Panatta,
Raffaella Santoro

Affiliations

Cristiana Bersaglieri: Department of Molecular Mechanisms of Disease, DMMD, University of Zurich
Jelena Kresoja-Rakic: Department of Molecular Mechanisms of Disease, DMMD, University of Zurich
Shivani Gupta: Department of Molecular Mechanisms of Disease, DMMD, University of Zurich
Dominik Bär: Department of Molecular Mechanisms of Disease, DMMD, University of Zurich
Rostyslav Kuzyakiv: Department of Molecular Mechanisms of Disease, DMMD, University of Zurich
Martina Panatta: Department of Molecular Mechanisms of Disease, DMMD, University of Zurich
Raffaella Santoro: Department of Molecular Mechanisms of Disease, DMMD, University of Zurich

DOI: https://doi.org/10.1038/s41467-022-29146-2
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 18

Abstract

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Mapping nucleolar-associated domains (NADs) is challenging as the nucleolus is membrane-less. Here the authors adapted the DNA adenine methyltransferase identification (DamID) method by engineering a “nucleolar histone” that can mark genomic contacts with the nucleolus through DNA methylation (m6A) and characterized NADs in mouse embryonic stem cells (ESCs) and derived neural progenitors (NPCs).

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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