Abiotic stress treatment reveals expansin like A gene OfEXLA1 improving salt and drought tolerance of Osmanthus fragrans by responding to abscisic acid

Bin Dong; Qianqian Wang; Dan Zhou; Yiguang Wang; Yunfeng Miao; Shiwei Zhong; Qiu Fang; Liyuan Yang; Zhen Xiao; Hongbo Zhao

Horticultural Plant Journal (Mar 2024)

Abiotic stress treatment reveals expansin like A gene OfEXLA1 improving salt and drought tolerance of Osmanthus fragrans by responding to abscisic acid

Bin Dong,
Qianqian Wang,
Dan Zhou,
Yiguang Wang,
Yunfeng Miao,
Shiwei Zhong,
Qiu Fang,
Liyuan Yang,
Zhen Xiao,
Hongbo Zhao

Affiliations

Bin Dong: School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Hangzhou, Zhejiang 311300, China; Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Hangzhou, Zhejiang 311300, China
Qianqian Wang: School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Hangzhou, Zhejiang 311300, China
Dan Zhou: School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Hangzhou, Zhejiang 311300, China
Yiguang Wang: School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Hangzhou, Zhejiang 311300, China
Yunfeng Miao: School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Hangzhou, Zhejiang 311300, China
Shiwei Zhong: School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Hangzhou, Zhejiang 311300, China; Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Hangzhou, Zhejiang 311300, China
Qiu Fang: School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Hangzhou, Zhejiang 311300, China; Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Hangzhou, Zhejiang 311300, China
Liyuan Yang: School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Hangzhou, Zhejiang 311300, China; Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Hangzhou, Zhejiang 311300, China
Zhen Xiao: School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Hangzhou, Zhejiang 311300, China; Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Hangzhou, Zhejiang 311300, China
Hongbo Zhao: School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Hangzhou, Zhejiang 311300, China; Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Hangzhou, Zhejiang 311300, China; Corresponding author.

Journal volume & issue: Vol. 10, no. 2
pp. 573 – 585

Abstract

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Sweet osmanthus (Osmanthus fragrans) is a having general approval aromatic tree in China that is widely applied to landscaping and gardening. However, the evergreen tree adaptability is limited by many environmental stresses. Currently, limited information is available regarding the genetic analysis and functional identification of expansin genes in response to abiotic stress in sweet osmanthus. In this study, a total of 29 expansin genes were identified and divided into four groups by genome-wide analysis from the sweet osmanthus genome. Transcriptome and quantitative Real-time PCR analysis showed that the cell wall-localized protein expansin-like A (OfEXLA1) gene was significantly induced by salt and drought treatment. Histochemical GUS staining of transgenic Arabidopsis lines in which GUS activity was driven with the OfEXLA1 promoter, GUS activity was significantly induced by salt, drought, and exogenous abscisic acid (ABA). In yeast, we found OfEXLA1 overexpression significantly improved the population of cells compared with wild-type strains after NaCl and polyethylene glycol (PEG) treatment. Additionally, OfEXLA1 overexpression not only promoted plant growth, but also improved the salt and drought tolerance in Arabidopsis. To gain insight into the role of ABA signaling in the regulation of OfEXLA1 improving abiotic tolerance in sweet osmanthus, four differentially expressed ABA Insensitive 5 (ABI5)-like genes (OfABL4, OfABL5, OfABL7, and OfABL8) were identified from transcriptome, and dual-luciferase (dual-LUC) and yeast one hybrid (Y1H) assay showed that OfABL4 and OfABL5 might bind to OfEXLA1 promoter to accumulate the OfEXLA1 expression by responding to ABA signaling to improve abiotic tolerance in sweet osmanthus. These results provide the information for understanding the molecular functions of expansin-like A gene and molecular breeding of sweet osmanthus in future.

Published in Horticultural Plant Journal

ISSN: 2095-9885 (Print); 2468-0141 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Plant culture
Website: https://www.keaipublishing.com/en/journals/horticultural-plant-journal/

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