Emerging Infectious Diseases (May 2019)

Novel Sequence Type in Bacillus cereus Strains Associated with Nosocomial Infections and Bacteremia, Japan

  • Reiko Akamatsu,
  • Masato Suzuki,
  • Keiji Okinaka,
  • Teppei Sasahara,
  • Kunikazu Yamane,
  • Satowa Suzuki,
  • Daisuke Fujikura,
  • Yoshikazu Furuta,
  • Naomi Ohnishi,
  • Minoru Esaki,
  • Keigo Shibayama,
  • Hideaki Higashi

DOI
https://doi.org/10.3201/eid2505.171890
Journal volume & issue
Vol. 25, no. 5
pp. 883 – 890

Abstract

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Bacillus cereus is associated with foodborne illnesses characterized by vomiting and diarrhea. Although some B. cereus strains that cause severe extraintestinal infections and nosocomial infections are recognized as serious public health threats in healthcare settings, the genetic backgrounds of B. cereus strains causing such infections remain unknown. By conducting pulsed-field gel electrophoresis and multilocus sequence typing, we found that a novel sequence type (ST), newly registered as ST1420, was the dominant ST isolated from the cases of nosocomial infections that occurred in 3 locations in Japan in 2006, 2013, and 2016. Phylogenetic analysis showed that ST1420 strains belonged to the Cereus III lineage, which is much closer to the Anthracis lineage than to other Cereus lineages. Our results suggest that ST1420 is a prevalent ST in B. cereus strains that have caused recent nosocomial infections in Japan.

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