Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells

Dacheng Ma; Shuguang Peng; Zhen Xie

doi:10.1038/ncomms13056

Nature Communications (Oct 2016)

Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells

Dacheng Ma,
Shuguang Peng,
Zhen Xie

Affiliations

Dacheng Ma: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Department of Automation, Center for Synthetic and System Biology, Tsinghua National Lab for Information Science and Technology, Tsinghua University
Shuguang Peng: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Department of Automation, Center for Synthetic and System Biology, Tsinghua National Lab for Information Science and Technology, Tsinghua University
Zhen Xie: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Department of Automation, Center for Synthetic and System Biology, Tsinghua National Lab for Information Science and Technology, Tsinghua University

DOI: https://doi.org/10.1038/ncomms13056
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 7

Abstract

Read online

Molecular engineering of Cas9 has the potential to expand the application of CRISPR-Cas technology. Here, Ma et al. show that dCas9 can be split and reconstituted in human cells and use a domain swapping strategy to engineer custom Cas9-based logic circuits and sensory switches.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal