Machine Learning Technology for EEG-Forecast of the Blood–Brain Barrier Leakage and the Activation of the Brain’s Drainage System during Isoflurane Anesthesia
Oxana Semyachkina-Glushkovskaya,
Konstantin Sergeev,
Nadezhda Semenova,
Andrey Slepnev,
Anatoly Karavaev,
Alexey Hramkov,
Mikhail Prokhorov,
Ekaterina Borovkova,
Inna Blokhina,
Ivan Fedosov,
Alexander Shirokov,
Alexander Dubrovsky,
Andrey Terskov,
Maria Manzhaeva,
Valeria Krupnova,
Alexander Dmitrenko,
Daria Zlatogorskaya,
Viktoria Adushkina,
Arina Evsukova,
Matvey Tuzhilkin,
Inna Elizarova,
Egor Ilyukov,
Dmitry Myagkov,
Dmitry Tuktarov,
Jürgen Kurths
Affiliations
Oxana Semyachkina-Glushkovskaya
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Konstantin Sergeev
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Nadezhda Semenova
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Andrey Slepnev
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Anatoly Karavaev
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Alexey Hramkov
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Mikhail Prokhorov
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Ekaterina Borovkova
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Inna Blokhina
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Ivan Fedosov
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Alexander Shirokov
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Alexander Dubrovsky
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Andrey Terskov
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Maria Manzhaeva
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Valeria Krupnova
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Alexander Dmitrenko
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Daria Zlatogorskaya
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Viktoria Adushkina
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Arina Evsukova
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Matvey Tuzhilkin
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Inna Elizarova
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Egor Ilyukov
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Dmitry Myagkov
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Dmitry Tuktarov
Institute of Physics, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Jürgen Kurths
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Anesthesia enables the painless performance of complex surgical procedures. However, the effects of anesthesia on the brain may not be limited only by its duration. Also, anesthetic agents may cause long-lasting changes in the brain. There is growing evidence that anesthesia can disrupt the integrity of the blood–brain barrier (BBB), leading to neuroinflammation and neurotoxicity. However, there are no widely used methods for real-time BBB monitoring during surgery. The development of technologies for an express diagnosis of the opening of the BBB (OBBB) is a challenge for reducing post-surgical/anesthesia consequences. In this study on male rats, we demonstrate a successful application of machine learning technology, such as artificial neural networks (ANNs), to recognize the OBBB induced by isoflurane, which is widely used in surgery. The ANNs were trained on our previously presented data obtained on the sound-induced OBBB with an 85% testing accuracy. Using an optical and nonlinear analysis of the OBBB, we found that 1% isoflurane does not induce any changes in the BBB, while 4% isoflurane caused significant BBB leakage in all tested rats. Both 1% and 4% isoflurane stimulate the brain’s drainage system (BDS) in a dose-related manner. We show that ANNs can recognize the OBBB induced by 4% isoflurane in 57% of rats and BDS activation induced by 1% isoflurane in 81% of rats. These results open new perspectives for the development of clinically significant bedside technologies for EEG-monitoring of OBBB and BDS.
