Mitochondria Specific Antioxidant, MitoTEMPO, Modulates Cd Uptake and Oxidative Response of Soybean Seedlings
Dalir Fayazipour,
Joanna Deckert,
Gholamali Akbari,
Elias Soltani,
Jagna Chmielowska-Bąk
Affiliations
Dalir Fayazipour
Department of Agronomy and Plant Breeding Sciences, College of Aboureihan, University of Tehran, Tehran P.O. Box 3391653775, Iran
Joanna Deckert
Department of Plant Ecophysiology, Institute of Experimental Biology, Faculty of Biology, School of Natural Sciences, Adam Mickiewicz University, 61-712 Poznań, Poland
Gholamali Akbari
Department of Agronomy and Plant Breeding Sciences, College of Aboureihan, University of Tehran, Tehran P.O. Box 3391653775, Iran
Elias Soltani
Department of Agronomy and Plant Breeding Sciences, College of Aboureihan, University of Tehran, Tehran P.O. Box 3391653775, Iran
Jagna Chmielowska-Bąk
Department of Plant Ecophysiology, Institute of Experimental Biology, Faculty of Biology, School of Natural Sciences, Adam Mickiewicz University, 61-712 Poznań, Poland
Numerous reports find that Cd induces formation of reactive oxygen species (ROS) in plants. However, a general ROS pool is usually studied, without distinction of their production site. In the present study, we applied a mitochondria-specific antioxidant, MitoTEMPO, to elucidate the role of mitochondria-derived ROS in the response of soybean seedlings to short-term (48 h) Cd stress. The obtained results showed that Cd caused a reduction in root length and fresh weight and increase in the level of superoxide anion, hydrogen peroxide, markers of lipid peroxidation (thiobarbituric reactive substances, TBARS) and markers of RNA oxidation (8-hydroxyguanosine, 8-OHG) in seedling roots. Application of MitoTEMPO affected Cd uptake in a dose-dependent manner and diminished the Cd-dependent induction of superoxide anion and lipid peroxidation.
