Antibacterial and Phytochemical Screening of Artemisia Species
Maria-Evelina Bordean,
Rodica Ana Ungur,
Dan Alexandru Toc,
Ileana Monica Borda,
Georgiana Smaranda Marțiș,
Carmen Rodica Pop,
Miuța Filip,
Mihaela Vlassa,
Bogdana Adriana Nasui,
Anamaria Pop,
Delia Cinteză,
Florina Ligia Popa,
Sabina Marian,
Lidia Gizella Szanto,
Sevastița Muste
Affiliations
Maria-Evelina Bordean
Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 64 Calea Floresti, 400509 Cluj-Napoca, Romania
Rodica Ana Ungur
Department of Medical Specialties, Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, Romania
Dan Alexandru Toc
Department of Microbiology, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania
Ileana Monica Borda
Department of Medical Specialties, Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, Romania
Georgiana Smaranda Marțiș
Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 64 Calea Floresti, 400509 Cluj-Napoca, Romania
Carmen Rodica Pop
Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 64 Calea Floresti, 400509 Cluj-Napoca, Romania
Miuța Filip
Raluca Ripan Institute for Research in Chemistry, Babeș-Bolyai University, 30 Fântânele Street, 400294 Cluj-Napoca, Romania
Mihaela Vlassa
Raluca Ripan Institute for Research in Chemistry, Babeș-Bolyai University, 30 Fântânele Street, 400294 Cluj-Napoca, Romania
Bogdana Adriana Nasui
Department of Community Health, “Iuliu Hațieganu” University of Medicine and Pharmacy, 6 Louis Pasteur Street, 400349 Cluj-Napoca, Romania
Anamaria Pop
Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 64 Calea Floresti, 400509 Cluj-Napoca, Romania
Delia Cinteză
9th Department-Physical Medicine and Rehabilitation, Carol Davila Univerity of Medicine and Pharmacy, 050474 București, Romania
Florina Ligia Popa
Physical Medicine and Rehabilitation Department, Faculty of Medicine, “Lucian Blaga” University of Sibiu, Victoriei Blvd., 550024 Sibiu, Romania
Sabina Marian
Faculty of Biology and Geology, Babeș-Bolyai University, 44 Republicii Street, 400015 Cluj-Napoca, Romania
Lidia Gizella Szanto
Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 64 Calea Floresti, 400509 Cluj-Napoca, Romania
Sevastița Muste
Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 64 Calea Floresti, 400509 Cluj-Napoca, Romania
Taking into account the increasing number of antibiotic-resistant bacteria, actual research focused on plant extracts is vital. The aim of our study was to investigate leaf and stem ethanolic extracts of Artemisia absinthium L. and Artemisia annua L. in order to explore their antioxidant and antibacterial activities. Total phenolic content (TPC) was evaluated spectrophotometrically. Antioxidant activity was evaluated by DPPH and ABTS. The antibacterial activity of wormwood extracts was assessed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Salmonella enteritidis cultures, and by zone of inhibition in Klebsiella carbapenem-resistant enterobacteriaceae (CRE) and Escherichia coli extended-spectrum β-lactamases cultures (ESBL). The Artemisia annua L. leaf extract (AnL) exhibited the highest TPC (518.09 mg/mL) and the highest expression of sinapic acid (285.69 ± 0.002 µg/mL). Nevertheless, the highest antioxidant capacity (1360.51 ± 0.04 µM Trolox/g DW by ABTS and 735.77 ± 0.02 µM Trolox/g DW by DPPH) was found in Artemisia absinthium L. leaf from the second year of vegetation (AbL2). AnL extract exhibited the lowest MIC and MBC for all tested bacteria and the maximal zone of inhibition for Klebsiella CRE and Escherichia coli ESBL. Our study revealed that AbL2 exhibited the best antioxidant potential, while AnL extract had the strongest antibacterial effect.
