The Anti-Proliferative Activity of Secondary Metabolite from the Marine <i>Streptomyces</i> sp. against Prostate Cancer Cells
Hung-Yu Lin,
Yong-Shiou Lin,
Shou-Ping Shih,
Sung-Bau Lee,
Mohamed El-Shazly,
Ken-Ming Chang,
Yu-Chen S. H. Yang,
Yi-Lun Lee,
Mei-Chin Lu
Affiliations
Hung-Yu Lin
School of Medicine, College of Medicine, I-SHOU University, Division of Urology, Department of Surgery, E-Da Cancer & E-Da Hospital, Kaohsiung 824, Taiwan
Yong-Shiou Lin
Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan
Shou-Ping Shih
Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
Sung-Bau Lee
Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy, Taipei Medical University, Taipei 115, Taiwan
Mohamed El-Shazly
Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity Street, Cairo 115, Egypt
Ken-Ming Chang
Department of Pharmacy, Tajen University, Pingtung 907, Taiwan
Yu-Chen S. H. Yang
Joint Biobank, Office of Human Research, Taipei Medical University, Taipei 110, Taiwan
Yi-Lun Lee
Department of Urology, Pingtung Hospital, Ministry of Health and Welfare, Pingtung 944, Taiwan
Mei-Chin Lu
Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan
Many active substances from marine organisms are produced by symbiotic microorganisms such as bacteria, fungi, and algae. Secondary metabolites from marine actinomycetes exhibited several biological activities and provided interesting drug leads. This study reported the isolation of Lu01-M, a secondary metabolite from the marine actinomycetes Streptomyces sp., with potent anti-proliferative activity against prostate cancers. Lu01-M blocked cell proliferation with IC50 values of 1.03 ± 0.31, 2.12 ± 0.38, 1.27 ± 0.25 μg/mL in human prostate cancer PC3, DU145, and LNCaP cells, respectively. Lu01-M induced cytotoxic activity through multiple mechanisms including cell apoptosis, necroptosis, autophagy, ER stress, and inhibiting colony formation and cell migration. Lu01-M induced cell cycle arrest at the G2/M phase and DNA damage. However, the activity of autophagy induced survival response in cancer cells. Our findings suggested that Lu01-M holds the potential to be developed as an anti-cancer agent against prostate cancers.
