A Europium Nanosphere-Based Time-Resolved Fluorescent Immunochromatographic Assay for the Rapid Screening of 4,4′-Dinitrocarbanilide: Aiming at Improving Strip Method Performance
Ming Zou,
Yongkang Yin,
Liuchuan Guo,
Qidi Zhang,
Jinyan Li,
Hong Zhang,
Qian Song,
Zhaojie Li,
Li Wang,
Xiang Ao,
Xiao Liang
Affiliations
Ming Zou
College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
Yongkang Yin
College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
Liuchuan Guo
College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
Qidi Zhang
College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
Jinyan Li
College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
Hong Zhang
College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
Qian Song
College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
Zhaojie Li
College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
Li Wang
College of Food Science and Engineering, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
Xiang Ao
Basic Medical College, Qingdao University, No. 308 Ningxia Road, Qingdao 266071, China
Xiao Liang
College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
Considering that the strip method is simple and convenient for users, a Europium nanosphere-based time-resolved fluorescent immunochromatographic assay (TRFICA) for the rapid screening of 4,4′-dinitrocarbanilide (DNC) was developed to improve the performance of strip assays. After optimization, TRFICA showed IC50, the limit of detection, and cut-off values of 0.4, 0.07, and 5.0 ng mL−1, respectively. No significant cross-reactivity (CR < 0.1%) with 15 DNC analogs was observed in the developed method. TRFICA was validated for DNC detection in spiked chicken homogenates, and recoveries ranged from 77.3% to 92.7%, with coefficients of variation of <14.9%. Moreover, the time needed for the detection procedure, including the sample pre-treatment, was less than 30 min for TRFICA, which had never been achieved before in other immunoassays. The newly developed strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique for DNC analysis in chicken muscle.
