Food and Agricultural Immunology (Jan 2018)

Immunochromatographic strip for ultrasensitive detection of fumonisin B1

  • Kai Hao,
  • Steven Suryoprabowo,
  • Tao Hong,
  • Shanshan Song,
  • Liqiang Liu,
  • Qiankun Zheng,
  • Hua Kuang

DOI
https://doi.org/10.1080/09540105.2018.1439455
Journal volume & issue
Vol. 29, no. 1
pp. 699 – 710

Abstract

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A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection of FB1 in corn samples. Under the optimized condition, the cut-off limits of test strips for FB1 were found to be 50 ng/mL in 0.01 M PBS and 25 ng/mL in corn samples. Results could be obtained within 5 min. The results revealed that the developed method is a rapid, sensitive, and simple tool for the detection of FB1.

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