COVID (Jul 2023)
Extraction-Free RT-PCR Surveillance Testing and Reporting for SARS-CoV-2
- Patrick R. Carney,
- Tyler Duellman,
- Jia-Yi Chan,
- Lauren Wells,
- Michael Tessmer,
- Leah Frater-Rubsam,
- Molly Zeller,
- Mark Field,
- James Speers,
- Kelly Tyrrell,
- Luke Thompson,
- Michael Bondurant,
- Tami Morin,
- Tamra Dagnon,
- Brian Goff,
- Corissa Runde,
- Sandra Splinter-Bondurant,
- Charles Konsitzke,
- Patrick Kelly,
- Christopher A. Bradfield,
- Joshua Hyman
Affiliations
- Patrick R. Carney
- Molecular and Environmental Toxicology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, USA
- Tyler Duellman
- Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, USA
- Jia-Yi Chan
- Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, USA
- Lauren Wells
- Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, USA
- Michael Tessmer
- Division of Information Technology, University of Wisconsin-Madison, Madison, WI 53706, USA
- Leah Frater-Rubsam
- Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, USA
- Molly Zeller
- Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, USA
- Mark Field
- Division of Information Technology, University of Wisconsin-Madison, Madison, WI 53706, USA
- James Speers
- Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, USA
- Kelly Tyrrell
- University Communications, University of Wisconsin-Madison, Madison, WI 53706, USA
- Luke Thompson
- University Health Services, University of Wisconsin-Madison, Madison, WI 53706, USA
- Michael Bondurant
- Division of Information Technology, University of Wisconsin-Madison, Madison, WI 53706, USA
- Tami Morin
- University Health Services, University of Wisconsin-Madison, Madison, WI 53706, USA
- Tamra Dagnon
- University Health Services, University of Wisconsin-Madison, Madison, WI 53706, USA
- Brian Goff
- University Health Services, University of Wisconsin-Madison, Madison, WI 53706, USA
- Corissa Runde
- Division of Information Technology, University of Wisconsin-Madison, Madison, WI 53706, USA
- Sandra Splinter-Bondurant
- Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, USA
- Charles Konsitzke
- Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, USA
- Patrick Kelly
- University Health Services, University of Wisconsin-Madison, Madison, WI 53706, USA
- Christopher A. Bradfield
- Molecular and Environmental Toxicology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, USA
- Joshua Hyman
- Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, USA
- DOI
- https://doi.org/10.3390/covid3070075
- Journal volume & issue
-
Vol. 3,
no. 7
pp. 1031 – 1041
Abstract
The COVID-19 pandemic necessitated sensitive, fast, and inexpensive testing for the virus in 2020 prior to the widespread availability of vaccines. Early testing efforts were limited by bottlenecks on reagents, low-throughput testing options, and the slow return of test results. In this paper, we detail the testing pipeline we established at the University of Wisconsin-Madison for rapid, inexpensive, and sensitive surveillance testing for SARS-CoV-2, and we highlight the strengths of the platform that would allow it to be applied to other disease surveillance projects, SARS-CoV-2 variant testing, or future pandemics. This pipeline can be quickly established for further accreditation and clinical application.
Keywords
