Polyphosphate Ester-Type Transporters Improve Antimicrobial Properties of Oxytetracycline
Mariya Kozak,
Anna Stasiuk,
Vasyl Vlizlo,
Dmytro Ostapiv,
Yulia Bodnar,
Nataliia Kuz’mina,
Natalia Figurka,
Natalia Nosova,
Roman Ostapiv,
Igor Kotsumbas,
Sergiy Varvarenko,
Volodymyr Samaryk
Affiliations
Mariya Kozak
Institute of Animal Biology of the NAAS (National Academy of Agrarian Sciences) of Ukraine, 79034 Lviv, Ukraine
Anna Stasiuk
Department of Organic Chemistry, Lviv Polytechnic National University, 79000 Lviv, Ukraine
Vasyl Vlizlo
Department of Internal Animal Diseases and Clinical Diagnostics, S. Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, 79010 Lviv, Ukraine
Dmytro Ostapiv
Institute of Animal Biology of the NAAS (National Academy of Agrarian Sciences) of Ukraine, 79034 Lviv, Ukraine
Yulia Bodnar
Institute of Animal Biology of the NAAS (National Academy of Agrarian Sciences) of Ukraine, 79034 Lviv, Ukraine
Nataliia Kuz’mina
Institute of Animal Biology of the NAAS (National Academy of Agrarian Sciences) of Ukraine, 79034 Lviv, Ukraine
Natalia Figurka
Department of Organic Chemistry, Lviv Polytechnic National University, 79000 Lviv, Ukraine
Natalia Nosova
Department of Organic Chemistry, Lviv Polytechnic National University, 79000 Lviv, Ukraine
Roman Ostapiv
State Research Control Institute of Veterinary Medicinal Products and Feed Additives, 79019 Lviv, Ukraine
Igor Kotsumbas
State Research Control Institute of Veterinary Medicinal Products and Feed Additives, 79019 Lviv, Ukraine
Sergiy Varvarenko
Department of Organic Chemistry, Lviv Polytechnic National University, 79000 Lviv, Ukraine
Volodymyr Samaryk
Department of Organic Chemistry, Lviv Polytechnic National University, 79000 Lviv, Ukraine
Prolonged use of antibiotics can cause toxicity in human and animal cells and lead to the development of antibiotic resistance. The development of drug delivery systems for enhanced antibacterial properties of antibiotics could reduce toxic effects and minimize the development of resistance. The aim of this study was to evaluate the effectiveness of oxytetracycline in complexes with new polyphosphate ester-type transporters and to investigate the antimicrobial effect of these complexes on Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus growth in vitro. Two polyphosphate ester-type transporters with different molecular weights were synthesized, and oxytetracycline was attached through the phosphorus groups. To determine the sensitivities of microorganisms, oxytetracycline hydrochloride and oxytetracycline complexes with polyphosphate ester-type transporters (P4 and P6) were added to liquid and solid media with E. coli, P. aeruginosa, and S. aureus in different doses. Oxytetracycline in complex with polyphosphate ester-type transporters at low doses (2.3 to 3.8 μg/disk or μg/mL) in both solid and liquid media inhibits the growth of S. aureus more effectively than oxytetracycline alone. The maximum influence on E. coli growth on solid media is observed at a dose of 8 μg/disk of oxytetracycline in combination with both P4 and P6 polyphosphate ester-type transporters. P. aeruginosa growth under the influence of oxytetracycline in combination with polyphosphate-ester type transporters in a liquid medium depends on the dose of antibiotic and the day of cultivation.
