Defoliation of Tilia cordata trees associated with Apiognomonia errabunda infection in Finland

Abstract

We investigated the causative agent of a disease outbreak affecting small-leaved limes ( Mill.) and resulting in darkening of the leaf petioles and excessive defoliation during summer 2016 in southern Finland. The fungal species composition of the symptomatic petioles was examined by culture isolation and molecular identification using ITS rDNA sequences, which revealed the most prevalent fungal species present in the petioles as (Roberge) Höhn. Based on reviewing curated herbarium specimens deposited at the Universities of Helsinki and Turku, is native and widely distributed in small-leaved limes in Finland, and occasionally infects also other broadleaved trees, including L. and ornamental species of L. and L. The ITS sequence analysis conducted during this study revealed minor within-species polymorphisms similar to those observed earlier in the Central European and Russian populations of , and reports the first nucleotide sequences of this species from the Nordic countries.Tilia cordataApiognomonia errabundaA. errabundaQuercus roburTiliaFagusA. errabunda