Epstein-Barr Virus Latent Membrane Protein-1 Induces the Expression of SUMO-1 and SUMO-2/3 in LMP1-positive Lymphomas and Cells

Scientific Reports. 2019;9(1):1-13 DOI 10.1038/s41598-018-36312-4

 

Journal Homepage

Journal Title: Scientific Reports

ISSN: 2045-2322 (Online)

Publisher: Nature Publishing Group

LCC Subject Category: Medicine | Science

Country of publisher: United Kingdom

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS

Sadia Salahuddin (Departments of Medicine and Microbiology and Immunology, The University of North Carolina)
Emma K. Fath (Lineberger Comprehensive Cancer Center, The University of North Carolina)
Natalie Biel (Department of Basic Medical Sciences, Mercer University School of Medicine)
Ashley Ray (Department of Basic Medical Sciences, Mercer University School of Medicine)
C. Randall Moss (Department of Basic Medical Sciences, Mercer University School of Medicine)
Akash Patel (Department of Basic Medical Sciences, Mercer University School of Medicine)
Sheetal Patel (Department of Basic Medical Sciences, Mercer University School of Medicine)
Leslie Hilding (Department of Basic Medical Sciences, Mercer University School of Medicine)
Matthew Varn (Department of Basic Medical Sciences, Mercer University School of Medicine)
Tabithia Ross (Department of Basic Medical Sciences, Mercer University School of Medicine)
Wyatt T. Cramblet (Department of Basic Medical Sciences, Mercer University School of Medicine)
Angela Lowrey (Department of Basic Medical Sciences, Mercer University School of Medicine)
Joseph S. Pagano (Departments of Medicine and Microbiology and Immunology, The University of North Carolina)
Julia Shackelford (Department of Cellular Biology and Physiology, The University of North Carolina)
Gretchen L. Bentz (Lineberger Comprehensive Cancer Center, The University of North Carolina)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 20 weeks

 

Abstract | Full Text

Abstract Epstein-Barr Virus latent membrane protein-1 (LMP1) interacts with the SUMO-conjugating enzyme Ubc9, which induces protein sumoylation and may contribute to LMP1-mediated oncogenesis. After analyzing human lymphoma tissues and EBV-positive cell lines, we now document a strong correlation between LMP1 and sumo-1/2/3 or SUMO-1/2/3 levels, and show that LMP1-induced sumo expression requires the activation of NF-κB signaling through CTAR1 and CTAR2. Together, these results point to a second mechanism by which LMP1 dysregulates sumoylation processes and adds EBV-associated lymphomas to the list of malignancies associated with increased SUMO expression.