Dataset of amplicon metagenomic assessment of barley rhizosphere bacteria under different fertilization regimes

Abstract

The metagenomic dataset profiled in this research is built on bacterial 16S rRNA gene amplicon of DNA mined from barley rhizosphere under chemical (CB) and organic (OB) fertilization. Amplicon-based sequencing was prepared by the Illumina platform, and the raw sequence dataset was examined using Metagenomic Rast Server (MG-RAST). The metagenome comprised sixteen samples that include CB1 (494,583 bp), CB2 (586,532 bp), CB3 (706,685 bp), CB4 (574,606 bp), CB5 (395,460 bp), CB6 (520,822 bp), CB7 (511,729 bp), CB8 (548,074 bp), OB1 (642,794 bp), OB2 (513,767 bp), OB3 (461,293 bp), OB4 (498,241 bp), OB5 (689,497 bp), OB6 (423,436 bp), OB7 (478,657 bp) and OB8 (279,186 bp). Information from the metagenome sequences is accessible under the bioproject numbers PRJNA827679 (CB1), PRJNA827686 (CB2), PRJNA827693 (CB3), PRJNA827699 (CB4), PRJNA827706 (CB5), PRJNA827761 (CB6), PRJNA827780 (CB7), PRJNA827786 (CB8), PRJNA826806 (OB1), PRJNA826824 (OB2), PRJNA826834 (OB3), PRJNA826841 (OB4), PRJNA826853 (OB5), PRJNA827254 (OB6), PRJNA827256 (OB7), and PRJNA827257 (OB8) at NCBI. Actinobacteria dominated the soil samples at the phylum level.

Keywords

• Anthropogenic interference
• Food safety
• Metagenome
• MG-RAST
• Unclassified microbiome