Distribution of Epstein-Barr virus in the oral cavity of Thais with various oral mucosal conditions

Pasinee Vorakulpipat; Nakarin Kitkumthorn; Puangwan Lapthanasupkul; Dulyapong Rungraungrayabkul; Boworn Klongnoi; Siribang-on Piboonniyom Khovidhunkit

Heliyon (Jan 2024)

Distribution of Epstein-Barr virus in the oral cavity of Thais with various oral mucosal conditions

Pasinee Vorakulpipat,
Nakarin Kitkumthorn,
Puangwan Lapthanasupkul,
Dulyapong Rungraungrayabkul,
Boworn Klongnoi,
Siribang-on Piboonniyom Khovidhunkit

Affiliations

Pasinee Vorakulpipat: Department of Advanced General Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Nakarin Kitkumthorn: Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Puangwan Lapthanasupkul: Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Dulyapong Rungraungrayabkul: Department of Oral Medicine and Periodontology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Boworn Klongnoi: Development of Disease Management Model for Oral Cancer with an Integration Network of Screening, Surveillance, and Treatment from Primary Care Unit to Tertiary Care in North East Health District Project, Faculty of Dentistry, Mahidol University, Bangkok, Thailand; Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Siribang-on Piboonniyom Khovidhunkit: Department of Advanced General Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand; Development of Disease Management Model for Oral Cancer with an Integration Network of Screening, Surveillance, and Treatment from Primary Care Unit to Tertiary Care in North East Health District Project, Faculty of Dentistry, Mahidol University, Bangkok, Thailand; Corresponding author. Department of Advanced General Dentistry, Faculty of Dentistry, Mahidol University, 6 Yothi Rd. Rajthewee, Bangkok, 10400, Thailand.

Journal volume & issue: Vol. 10, no. 2
p. e24222

Abstract

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Objectives: We aimed to examine the presence of EBV, EBV strains, and variants among 3 oral conditions including normal oral mucosa (NOM), oral potentially malignant disorders/oral cancer (OPMDs/OC) and non-OPMDs/OC in a group of Thais. Material and methods: Oral exfoliated cells were obtained from 315 participants living in the northeastern and central regions of Thailand. The participants were divided into 3 groups encompassing the NOM, the OPMDs/OC and the non-OPMDs/OC groups. The presence of EBV was first determined by PCR using primers for LMP1 gene. Subsequently, EBV strains of EBNA3c and variants based on LMP1 sequences were determined by real-time PCR. Results: The prevalence of EBV in OPMDs/OC, non-OPMDs/OC and NOM were 72.0 %, 56.2 %, and 27.2 % respectively. EBV type A, B and AB were found in 52.1 %, 32.1 % and 15.8 % of all positive samples, respectively. The percentage of participants with EBV type A was more prominent in the NOM group (72.0 %) compared to the non-OPMDs/OC (54.8 %) and the OPMDs/OC group (41.8 %) whereas EBV type B was higher in the OPMDs/OC group (35.8 %) compared to the non-OPMDs/OC (31.5 %) and the NOM (24.0 %) groups. Regarding EBV variants, 30-bp deletion LMP1 variant (del-LMP1) which is more associated with malignant transformation was predominately found in the OPMDs/OC (32.8 %) and the non-OPMDs/OC (38.4 %) groups compared to the NOM group (20.0 %). Conclusions: High frequency of EBV was demonstrated in the OPMDs/OC group. EBV type A was more predominant in the NOM group whereas EBV type B was more prevalent in the OPMDs/OC group. The del-LMP1 variant was more common in the OPMDs/OC and the non-OPMDs/OC groups.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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