Data on characterization of glass biochips and validation of the label-free biosensor for detection of autoantibodies in human serum
A.V. Pushkarev,
A.V. Orlov,
S.L. Znoyko,
D.O. Novichikhin,
V.A. Bragina,
A.A. Sizikov,
E. Alipour,
H. Ghourchian,
A.I. Nikitin,
G.M. Sorokin,
B.G. Gorshkov,
P.I. Nikitin
Affiliations
A.V. Pushkarev
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskii per., Dolgoprudny, Moscow Region, 141700, Russia
A.V. Orlov
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskii per., Dolgoprudny, Moscow Region, 141700, Russia; Corresponding author: Alexey Orlov
S.L. Znoyko
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia
D.O. Novichikhin
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia
V.A. Bragina
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia
A.A. Sizikov
Moscow Institute of Physics and Technology, 9 Institutskii per., Dolgoprudny, Moscow Region, 141700, Russia
E. Alipour
Institute of Biochemistry and Biophysics (IBB), University of Tehran, P.O. Box 13145-1384, Tehran, Iran
H. Ghourchian
Institute of Biochemistry and Biophysics (IBB), University of Tehran, P.O. Box 13145-1384, Tehran, Iran
A.I. Nikitin
Volga branch of Moscow Automobile and Road State Technical University, Cheboksary 428000, Russia
G.M. Sorokin
Chuvash State University, 15 Moskovskij Ave., Cheboksary, 428015, Russia
B.G. Gorshkov
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia
P.I. Nikitin
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia
The data represent in-depth characterization of a novel method for highly sensitive simultaneous measuring in human serum of both critical parameters of autoantibodies: concentration and native kinetics. The latter refers to autoantibody interaction with free, not immobilized, antigen. The method and related biosensors are based on the spectral-correlation and spectral-phase interferometry. The data cover: multi-factor optimization and quantitative characterization of the developed affordable single-used biochips, including X-ray photoelectron spectroscopy (XPS) control of chemical modifications of the surface during fabrication; antibody screening; optimization and verification of protocols for label-free biosensing in human serum; mathematical model for fitting experimental data and calculation of kinetic constants of interaction of autoantibodies with free antigen; comprehensive verification of the method specificity; correlation between the data obtained with the developed biosensor and with enzyme linked immunosorbent assay (ELISA); comparison of analytical characteristics of the developed biosensor with the most advanced label-based methods. The data importance is confirmed by a companion paper (DOI 10.1016/j.bios.2020.112187), which shows that the combination of mentioned autoantibody parameters is promising for more accurate criteria for early diagnostics and efficient therapy of autoimmune disorders. The obtained data can be used in development of a wide range of biosensors, both label-free and based on various labels.
