BMC Veterinary Research (Aug 2012)

Modulation of ovine SBD-1 expression by 17beta-estradiol in ovine oviduct epithelial cells

  • Wen Shiyong,
  • Cao Guifang,
  • Bao Tuya,
  • Cheng LanLing,
  • Li Haijun,
  • Du Chenguang,
  • Tu Yong,
  • Li Qi,
  • Jian Ruizhen,
  • Zhao Pengwei,
  • Wuriliga

DOI
https://doi.org/10.1186/1746-6148-8-143
Journal volume & issue
Vol. 8, no. 1
p. 143

Abstract

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Abstract Background Mucosal epithelia, including those of the oviduct, secrete antimicrobial innate immune molecules (AIIMS). These have bactericidal/bacteriostatic functions against a variety of pathogens. Among the AIIMs, sheep β-defensin-1 (SBD-1) is one of the most potent. Even though the SBD-1 is an important AIIM and it is regulated closely by estrogenic hormone, the regulation mechanism of 17β-estradiol has not been clearly established. We investigated the effects of E2 and agonist or inhibitor on ovine oviduct epithelial cells in regard to SBD-1 expression using reverse transcription quantitative PCR (RT-qPCR). In addition, three different pathways were inhibited separately or simultaneously to confirm the effect of different inhibitors in the regulation mechanism. Results 17beta-estradiol (E2) induced release of SBD-1 in ovine oviduct epithelial cells. SBD-1 expression was mediated through G-protein-coupled receptor 30 (GPR30) and Estrogen Receptors (ERs) activation in ovine oviduct epithelial cell. Inhibition of gene expression of protein kinase A (PKA), protein kinase C (PKC), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) led to a decreased SBD-1 expression. Conclusions Taken together, E2-induced up-regulation of SBD-1 expressions were GPR30-dependent during prophase and ERs-dependent during later-stage in ovine oviduct epithelial cells, and we assume that the effect was completed by the PKA, PKC, and NF-κB pathways simultaneous.

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