Genomic Analysis of the ASMT Gene Family in Solanum lycopersicum
Weicheng Liu,
Dake Zhao,
Chunfang Zheng,
Chen Chen,
Xin Peng,
Yuan Cheng,
Hongjian Wan
Affiliations
Weicheng Liu
Zhengjiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Zhejiang Mariculture Research Institute, Wenzhou 325000, China
Dake Zhao
Laboratory of Ecology and Evolutionary Biology, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan University, Kunming 650091, China
Chunfang Zheng
Zhengjiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Zhejiang Mariculture Research Institute, Wenzhou 325000, China
Chen Chen
Zhengjiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Zhejiang Mariculture Research Institute, Wenzhou 325000, China
Xin Peng
Zhengjiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Zhejiang Mariculture Research Institute, Wenzhou 325000, China
Yuan Cheng
Statekey Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Hongjian Wan
Statekey Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Acetylserotonin methyltransferase (ASMT) is the last enzyme of melatonin biosynthesis and may play a rate-limiting role in the melatonin production of plants. In this study, systematic analysis of the ASMT gene family in tomato (Solanum lycopersicum Mill) has been presented by the integration of the structural features, phylogenetic relationships, exon/intron configuration, and expression profile during growth and development, as well as biotic stresses. The results revealed that the tomato genome encoded a minimum of 14 members, containing three probable encoded pseudogenes. Chromosome mapping indicated that the family had probably expanded via tandem duplication events. Genome-wide RNA-seq and qRT-PCR based gene expression analysis revealed that almost half of the SlASMT genes were expressed in at least one of the experimental stages studied and also showed differential accumulation. Furthermore, the tandem duplicated SlASMT genes showed differential expression levels, which indicated probable functional divergence during the course of the evolution. Finally, this study also determined that some SlASMT genes were induced by multiple pathogens. The results suggested that these genes could be involved in tomato plant response to biotic stresses.
