Repressing of SOX6 and SOX9 in Situ Chondrogenic Differentiation of Rat Bone Marrow Stromal Cells

Mohammad Hassan Karimfar; Aliasghar Peyvandi; Mohsen Noorozian; Navid Ahmadi Roozbahani; Reza Mastery Farahani; Maryam Sadat Khoramgah; Hadi Azimi; Ayad Bahadori Monfared; Hojjat-Allah Abbaszadeh

Anatomical Sciences Journal (May 2015)

Repressing of SOX6 and SOX9 in Situ Chondrogenic Differentiation of Rat Bone Marrow Stromal Cells

Mohammad Hassan Karimfar,
Aliasghar Peyvandi,
Mohsen Noorozian,
Navid Ahmadi Roozbahani,
Reza Mastery Farahani,
Maryam Sadat Khoramgah,
Hadi Azimi,
Ayad Bahadori Monfared,
Hojjat-Allah Abbaszadeh

Affiliations

Mohammad Hassan Karimfar: Department of Anatomical Sciences, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Aliasghar Peyvandi: Hearing Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohsen Noorozian: Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Navid Ahmadi Roozbahani: Hearing Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Reza Mastery Farahani: Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Maryam Sadat Khoramgah: Department of Medical Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hadi Azimi: Department of English Language Teaching, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Ayad Bahadori Monfared: Department of Health & Community Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hojjat-Allah Abbaszadeh: Department of Health & Community Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Journal volume & issue: Vol. 12, no. 2
pp. 75 – 82

Abstract

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Introduction: SOX9 is a transcriptional activator which is necessary for chondrogenesis. SOX6 are closely related to DNA-binding proteins that critically enhance its function. Therefore, to carry out the growth plate chondrocyte differentiation program, SOX9 and SOX6 collaborate genomewide. Chondrocyte differentiation is also known to be promoted by glucocorticoids through unknown molecular mechanisms. Methods: We investigated the effects of asynthetic glucocorticoid, dexamethasone (DEX), on SOX9 gene expression in chondrocytes. Results: SOX9 mRNA was expressed at high levels in these chondrocytes. Treatment with DEX resulted in enhancement of SOX9 mRNA expression. The DEX effect was dose dependent (0·5 nM and 1 nM). Conclusion: RT-PCR analysis revealed that DEX also enhanced the levels of SOX9 expression. It was observed that DEX had enhancing effect only on SOX9 the expression level was low for SOX6. It can thus be concluded that chondrocyte differentiation can be promoted by DEX via SOX9 enhancement.

Published in Anatomical Sciences Journal

ISSN: 2322-3626 (Print)
Publisher: Negah Institute for Scientific Communication
Country of publisher: Iran, Islamic Republic of
LCC subjects: Science: Human anatomy
Website: http://www.anatomyjournal.ir/

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