Разработка и регистрация лекарственных средств (Jan 2019)

DEVELOPMENT AND VALIDATION OF MOXIFLOXACIN DETERMINATION IN HUMAN PLASMA BY HPLC-UV METHOD

  • T. N. Komarov,
  • Yu. V. Medvedev,
  • I. E. Shohin,
  • Yu. E. Boldina,
  • A. A. Lvova,
  • E. S. Melnikov,
  • E. N. Fisher,
  • R. V. Ivanov,
  • R. J. Maksvitis

Journal volume & issue
Vol. 0, no. 3
pp. 174 – 179

Abstract

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A new method for determination of moxifloxacin in human plasma using HPLC with UV-detection is described. The sample preparation was made by protein precipitation by 50% trifluoracetic acid solution. The method was validated in terms of selectivity, calibration curve, accuracy, precision, lower limit of quantification, carry-over and stability. The analytical range was 100-5000 ng/mL. Limit of determination was 31 ng/mL. Method could be applied to moxifloxacin determination in plasma for PK and BE studies.

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