NUCLEOSIDE-DIPHOSPHATASE OF CESTODE BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE)

Abstract

Objective of research: To study the activities and properties of nucleoside- diphosphatase (NDPase) in cestode Bothriocephalus scorpii.Materials and methods: Cestodes were homogenized with 10 vol. of extraction medium. NDPase was detected in mitochondria and microsomes with substrates (IDP, GDP, UDP). Inorganic phosphorus was determined by the method of Kochetov (1980). The effects of 10 anthelmintic drugs on the activity of NDPase were studied.Results and discussion: It was found that the mitochondrial and microsomal fractions of cestodes B. scorpii have nucleoside diphosphatase activity.The activity of nucleoside diphosphatase depends on substrates and Mg2+ ions. The impact of various effectors and ions (Ca2+, Mn2+, Zn2+) on enzyme activity was determined.Effects of 10 anthelmintic drugs on activity of nucleoside diphosphatase were studied. The anthelmintics Bitionol and Trichlorophen have been proved effective.

Keywords

• nucleoside-diphosphatase
• inosine diphosphate
• uridine diphosphate
• guanosine diphosphate
• cestode
• mitochondria
• microsomes
• anthelmintic drugs