Breast Cancer: Targets and Therapy (Sep 2022)
Chloride Intracellular Channel 1 is a Potential Biomarker for Breast Cancer
Abstract
Jinwen Xia,1,2 Quhui Wang,1 Fei Ju,3 Xiang Luo,3 Feng Wang,4 Youlang Zhou,3 Hua Huang,5 Hua Wang,1,2 Xingli Bao6 1Department of Breast and Thyroid Surgery, Affiliated Hospital of Nantong University, Nantong, People’s Republic of China; 2Clinical Medicine, Medical College, Nantong University, Nantong, People’s Republic of China; 3Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, People’s Republic of China; 4Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, People’s Republic of China; 5Department of Pathology, Affiliated Hospital of Nantong University, Nantong, People’s Republic of China; 6Department of Medical Equipment, Affiliated Hospital of Nantong University, Nantong, People’s Republic of ChinaCorrespondence: Hua Wang, Department of Breast and Thyroid Surgery, Affiliated Hospital of Nantong University, No. 20, Xisi Road, Chongchuan District, Nantong City, Jiangsu Province, 226001, People’s Republic of China, Tel +86 137 062 92250, Email [email protected]: Multiple reports have demonstrated that highly expressed chloride intracellular channel 1 (CLIC1) exists in a range of malignant tumors and is involved in proliferation, invasion, and migration of cancer cells. There are few studies on CLIC1 and breast cancer (BC). The purpose of this research was to evaluate the expression level of CLIC1 in BC and its impact on prognosis of BC patients.Patients and Methods: Differences in CLIC1 expression levels in 25 pairs of BC and corresponding paracancerous specimens were tested by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot (WB). Immunohistochemistry (IHC) was performed to discuss the relevance between CLIC1 expression in BC tissue chips and clinicopathological parameters of BC patients. The effect of CLIC1 expression on patient prognosis was evaluated by Kaplan–Meier survival curve and Cox regression analysis. Receiver operating characteristic (ROC) curve assessed the diagnostic performance of CLIC1 for BC.Results: The experimental results of qRT-PCR and WB demonstrated that CLIC1 was highly expressed in BC tissues. IHC results showed that overexpression of CLIC1 was strictly correlated with tumor size, TNM classification, pathological grade, lymph node metastasis and Ki67. Patients with lower CLIC1 expression had longer overall survival (OS) and progression-free survival (PFS). Cox regression analysis and ROC curve confirmed that CLIC1 could independently influence the prognosis of BC patients and might have diagnostic efficiency.Conclusion: Overexpressed CLIC1 is closely related to the progression of BC and the poor prognosis of the patients, suggesting that it may act as a potential biological diagnostic index for BC.Keywords: CLIC1, clinical pathological parameter, prognosis, diagnosis
