Biotechnologia Acta (Jun 2016)

COMPLEXES OF BISCITRATOGERMANATES AND BISCITRATOSTANATES WITH METALS ARE MODIFIERS OF ACTIVITY OF Bacillus thuringiensis var. іsraelensis PEPTIDASES AND α-Penicillium canescens, Cladosporium cladosporioides AND Aspergillus niger GALACTOIDASES

  • L. D. Varbanets,
  • N. A. Nidialkova,
  • N. V. Borzova,
  • I. Yi. Sayfulina ,
  • O. E. Marcinko,
  • O. A. Chebanenko

DOI
https://doi.org/10.15407/biotech9.03.052
Journal volume & issue
Vol. 9, no. 3
pp. 52 – 60

Abstract

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The aim of the research was to study the effect of a number of coordination compounds of stanum and germanium (compounds 1‒8) as modifiers of activity of peptidases and α-galactosidases. The coordination compounds with the same type structure of were investigated as enzymes effectors. Two types of complexes: 1) [M(H2O)6][Ge(НCitr)2]4H2O (M = Mg(1), Mn(2), Co(3), Ni(4), Zn(5)), containing biscitrate-stanate anion ([Ge(НCitr)2]2-), and 2) [M(H2O)6][Sn(НCitr)2]4H2O (M = Mg(6), Co(7), Ni(8)), containing biscitratostanate anion and various hexaaquacations ([M(H2O)6]2+, М= Mg, Mn, Co, Ni, Zn) were studied. It is shown that the compound 6 which is a biscitratostanate complex containing magnesium ions as metal, can be used for stimulation on 20‒25% of collagenase activity of B. thuringiensis var. israelensis IMV B-7465 peptidase 1 and peptidase 2. Compound 1 (biscitrate-stanate complex containing magnesium ions as metal) and 7 (biscitratostanate complex containing cobalt ions as metal) and compound 6 in a concentration of 0.001% are able to increase elastolytic activity of peptidase 1 on 55‒58%. However compound 7 has shown the greatest activating effect. It increased the elastolytic activity of peptidase 2 on 100‒140% (for both tested concentrations). This indicates that the compound 7 can further be used as of peptidase 2 elastolytic activity effector. In the study of effect of the considered coordination compounds on the activity of α-galactosidase of Penicillium canescens, Cladosporium cladosporioides and Aspergillus niger was found that when using a number of complexes (1‒2 and 4‒8), there is a slight increase on 12‒20% of enzyme activity of P. canescens, and the maximum effect (~20%, concentration 0.01%) was provided by complex 6.

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