Anti-oxidative, anti-inflammatory, and anti-estrogenic effects of mushroom Mycoamaranthus cambodgensis (Pat.) Trappe, S. Lumyong, P. Lumyong, Sanmee & Zhu L. Yang extract

Abstract

Mycoamaranthus cambodgensis (Pat.) Trappe, S. Lumyong, P. Lumyong, Sanmee & Zhu L. Yang (Hymenogasteraceae) is a wild edible mushroom found in the deciduous forests of Thailand. It is used as a folk medicine for postpartum recovery. This study aimed to investigate the anti-oxidative, anti-estrogenic, and anti-inflammatory activities of M. cambodgensis extract (MCE). The MCE showed high anti-oxidative activity. From the DPPH and FRAP methods, the 50% effective concentrations were 55.51±3.62 µg/mL and 166.64±11.43 µg/mg, respectively. The extract contained high total phenolic (113.80±3.13 mg tannic acid equivalent/g) and flavonoid contents (2.61±0.12 mg quercetin equivalent/g). The MCE decreased pro-inflammatory mediator gene expressions including COX-2, IL-1β, IL-6, iNOS, and TNF-α. In 17-estradiol (E2)-induced MCF-7 cells, the MCE at 12.5 µg/mL synergistically augmented the proliferative effect of E2, whereas MCE at 50‒100 µg/mL significantly suppressed this E2 effect. The potent anti-oxidant content and anti-estrogenic activity of MCE may correlate to its anti-inflammatory activity and traditional uses. This mushroom is a good candidate for further natural product development.

Keywords

• mycoamaranthus cambodgensis
• anti-oxidative activity
• anti-estrogenic activity
• anti-inflammatory activity