MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct
Dong Chen,
Pengbo Chang,
Peng Ding,
Shuang Liu,
Qi Rao,
Oseweuba Valentine Okoro,
Lingling Wang,
Lihong Fan,
Amin Shavandi,
Lei Nie
Affiliations
Dong Chen
Department of Orthopedics, Affiliated Hospital of Jianghan University, Wuhan 430015, China
Pengbo Chang
Zhengzhou Technical College, Zhengzhou 450121, China
Peng Ding
College of Life Sciences, Xinyang Normal University (XYNU), Xinyang 464000, China
Shuang Liu
School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China
Qi Rao
Department of Orthopedics, Wuhan Hanyang Hospital, Wuhan University of Science and Technology, Wuhan 430050, China
Oseweuba Valentine Okoro
Université libre de Bruxelles (ULB), École polytechnique de Bruxelles, 3BIO-BioMatter, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Belgium
Lingling Wang
Analysis & Testing Center, Xinyang Normal University, Xinyang 464000, China; Corresponding author.
Lihong Fan
School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China; Corresponding authors. School of Resources and Environmental Engineering, Wuhan University of Technology, 122 Luoshi Road, Wuhan, Hubei, 430070, China.
Amin Shavandi
Université libre de Bruxelles (ULB), École polytechnique de Bruxelles, 3BIO-BioMatter, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Belgium; Corresponding author. Université libre de Bruxelles (ULB), École polytechnique de Bruxelles, 3BIO-BioMatter, Avenue F.D. Roosevelt, 50-CP 165/61, 1050 Brussels, Belgium.
Lei Nie
College of Life Sciences, Xinyang Normal University (XYNU), Xinyang 464000, China; Université libre de Bruxelles (ULB), École polytechnique de Bruxelles, 3BIO-BioMatter, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Belgium; Corresponding authors. College of Life Sciences, Xinyang Normal University (XYNU), Xinyang 464000, China.
Repair of osteochondral defects and regeneration of cartilage is a major challenge. In this work, the mesenchymal stem cells (MSCs)-laden hydrogel was designed using silk fibroin (SF) and gelatin methacrylate (GelMA), to encapsulate platelet-rich plasma (PRP). Initially, GelMA was synthesized, and SF was prepared using silkworm cocoon, then MSCs-laden SF/GelMA (SG) hydrogel was fabricated. The physicochemical properties of the hydrogels were evaluated using Fourier-transform infrared spectroscopy, scanning electron microscope, and rheometry. After hydrogel preparation, the viability of MSCs in the hydrogels was investigated via CCK-8 analysis and fluorescent images. The MSCs-laden SG hydrogel containing PRP was subsequently injected into the cartilage defect area in Sprague Dawley rats. Hematoxylin and eosin (H&E), Masson staining, and Mankin scores evaluation confirmed the new cartilage formation in 8 weeks. The results presented in the study, therefore, showed that the prepared MSCs-laden SG hydrogel loaded with PRP has the potential for cartilage reconstruction, which is crucial to the treatment of knee osteoarthritis.