Real-time quantitative PCR for detection and identification of Actinobacillus pleuropneumoniae serotype 2

Abstract

Introduction: Porcine pleuropneumonia inflicts important economic losses on most commercial herds. Detection of subclinical or chronic infection in animals still remains a challenge, as isolation and identification of A. pleuropneumoniae serotypes is difficult and quantification of the bacteria on agar plates is often almost impossible. The aim of the study was to develop and evaluate a serotype-specific quantitative TaqMan probe-based PCR for detection of serotype 2 in pig lungs, tonsils, and nasal swabs.

Keywords

• pigs
• actinobacillus pleuropneumoniae
• identification
• quantitative real-time pcr
• taqman probe