International Journal of General Medicine (Feb 2022)

LINC01006 and miR-3199 Serve as Novel Markers of Poor Prognosis in Colon Cancer and Regulate Cell Proliferation, Migration and Invasion

  • Wu Y,
  • Yu B,
  • Li Y,
  • Yu F,
  • Li Z,
  • Chen D,
  • Jiang F,
  • Bo J,
  • Xue H,
  • Lv H,
  • Li H

Journal volume & issue
Vol. Volume 15
pp. 1677 – 1687

Abstract

Read online

Yaoqiang Wu,1 Bo Yu,2 Yaping Li,3 Fuxiang Yu,1 Zhongguo Li,1 Daxin Chen,1 Feng Jiang,1 Jianbo Bo,1 Hongwei Xue,1 Hongyang Lv,1 Haiyang Li1 1Department of General Surgery, Dandong First Hospital, Dandong, Liaoning, People’s Republic of China; 2Department of Gastroenterology, Dandong First Hospital, Dandong, Liaoning, People’s Republic of China; 3Department of Intensive Care Unit, Dandong First Hospital, Dandong, Liaoning, People’s Republic of ChinaCorrespondence: Yaoqiang Wu, Department of General Surgery, Dandong First Hospital, Dandong, Liaoning, People’s Republic of China, Tel/Fax + 86-415-2819133, Email [email protected]: Colon cancer is the most commonly diagnosed gastrointestinal cancer. This research intended to evaluate the prognostic values of LINC01006 and miR-3199 for colon cancer and their effects on cell physiology.Patients and Methods: LINC01006 and miR-3199 expression levels were determined by RT-qPCR. Patients’ 5-year cumulative survival rate was analyzed by Kaplan–Meier curves with the Log rank test. Chi-square test and multivariate Cox regression analysis were used to access the clinical significance. CCK-8 assay, transwell assay, and TUNEL assays were used to monitor the change of cell proliferation, invasion, migration, and apoptosis.Results: The expression level of LINC01006 was increased while miR-3199 was decreased in colon tissues and cells compared to normal ones. This dysregulated expression was correlated with T stage (P = 0.002) and N stage (P = 0.009). High LINC01006 level (HR = 4.048, 95%: 1.502– 10.911, P = 0.006) or low miR-3199 level (HR = 3.421, 95% CI: 1.254– 9.330, P = 0.016) was outstanding for predicting poor prognosis in patients with colon cancer. Downregulation of LINC01006 reduced cell proliferation, invasion, and migration but induced cell apoptosis (P < 0.05).Conclusion: LINC01006 knockdown showed anti-proliferative, anti-metastatic, and apoptotic-induced effects on colon cancer cells. This study contributes to research on promising prognostic biomarkers of colon cancer and might give way to further investigation of alternative tumor targets.Keywords: colon cancer, LINC01006, miR-3199, prognosis

Keywords