Spontaneous pneumococcal peritonitis diagnosed by qPCR
Daniel Jarovsky,
Thais Grodzicki Ambrus,
Mariana Galvão Gurgel,
Marcelo Jenné Mimica,
Mariana Volpe Arnoni,
Flávia Jacqueline Almeida,
Marco Aurélio Palazzi Sáfadi,
Eitan Naaman Berezin
Affiliations
Daniel Jarovsky
Pediatric Infectious Diseases Unit - Santa Casa de São Paulo, São Paulo, Brazil; Corresponding author at: Alameda Jau 585/121, Jardim Paulista, São Paulo, SP, 01420001, Brazil.
Thais Grodzicki Ambrus
Pediatric Infectious Diseases Unit - Santa Casa de São Paulo, São Paulo, Brazil
Mariana Galvão Gurgel
Pediatric Infectious Diseases Unit - Santa Casa de São Paulo, São Paulo, Brazil
Marcelo Jenné Mimica
Pediatric Infectious Diseases Unit - Santa Casa de São Paulo, São Paulo, Brazil
Mariana Volpe Arnoni
Hospital Infection Control Unit - Santa Casa de São Paulo, São Paulo, Brazil
Flávia Jacqueline Almeida
Pediatric Infectious Diseases Unit - Santa Casa de São Paulo, São Paulo, Brazil
Marco Aurélio Palazzi Sáfadi
Pediatric Infectious Diseases Unit - Santa Casa de São Paulo, São Paulo, Brazil
Eitan Naaman Berezin
Pediatric Infectious Diseases Unit - Santa Casa de São Paulo, São Paulo, Brazil
Spontaneous bacterial peritonitis is an uncommon manifestation of invasive pneumococcal disease and frequently occurs when an underlying hepatic disease is present. Bacterial identification through culture can be particularly challenging in patients with prior or concurrent antimicrobial use. DNA amplification detects very few copies of target DNA under ideal conditions in CSF or pleural effusion and, therefore, can be useful in selected infections. A culture-negative spontaneous pneumococcal peritonitis without preexisting peritoneal disease diagnosed by qPCR is herein described. Keywords: Streptococcus pneumoniae, Invasive pneumococcal disease, Spontaneous peritonitis, Real-time PCR, lytA primer