Preparation of Biotinylated and FITC-Labelled Phosphorylcholine Poly(acrylamide) Derivatives and Their Application for Protein Ligand-Binding Studies

Abstract

Biotin- and FITC-labelled water-soluble poly(acrylamide) derivatives of phosphorylcholine were prepared by coupling either maleinylated (a) or periodate-oxidized (b) L-glyceryl phosphorylcholine to poly-(acrylamide-allylamine) copolymer. Biotinylated phosphorylcholine poly(acrylamide) derivatives of both types were tested with Limulus polyphemus C-reactive protein and were used for the study of the phosphorylcholine-binding properties of boar seminal plasma proteins. Binding sites for phosphorylcholine on the surface of bull sperms were visualized using a FITC-labelled derivative of the ligand.